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Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoid Pseudomonas aeruginosa as an evidence of phosphofructokinase activity

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Abstract

Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-1,6-P2) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6-bisphosphate aldolase negative mutant of this organism produced Fru-1,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6-phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.

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Abbreviations

ALD:

Fru-1,6-P2 aldolse

DHAP:

dihydroxyacetone phosphate

F6P:

fructose 6-phosphate

G6P:

glucose 6-phosphate

Gly3P:

glyceraldehyde 3-phosphate

KDPG:

2-keto 3-deoxy 6-phosphogluconate

PFK:

fructose 6-phosphate kinase

PGI:

phosphoglucose isomerase

6PG:

6-phosphogluconate

References

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Banerjee, P.C. Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoid Pseudomonas aeruginosa as an evidence of phosphofructokinase activity. Arch. Microbiol. 144, 405–407 (1986). https://doi.org/10.1007/BF00409892

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Key words

  • Pseudomonas aeruginosa
  • Fructose 6-phosphate kinase
  • Fructose bisphosphate aldolase mutant
  • Phosphoglucose isomerase mutant
  • Fructose bisphosphate formation
  • Mucoid