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Archives of Microbiology

, Volume 144, Issue 4, pp 393–397 | Cite as

Effects of calcium channel blockers on phototaxis and motility of Chlamydomonas reinhardtii

  • Wilhelm Nultsch
  • Jürgen Pfau
  • Reiner Dolle
Original Papers

Abstract

The effects of the four calcium channel blockers flunarizine, verapamil, diltiazem and nimodipine on motility and phototaxis of Chlamydomonas reinhardtio have been tested with a fully automated and computerized population system. Flunarizine inhibits motility transiently by causing the detachement of the flagella which, however, are regenerated during some hours. Phototaxis is inhibited to the same extent, but this is simply the result of the decreased motility and, hence, a non-specific effect. Verapamil causes also a detachement of the flagella with following regeneration, but in addition motility and phototaxis are inhibited by this drug to different extents, indicating the involvement of calcium channels in both processes. Diltiazem and nimodipine inhibit phototaxis without impairing motility, indicating that both processes are regulated in different ways. If diltiazem and nimodipine are applied simultaneously, no additive inhibitory effect can be observed. However, the combination of both blockers with verapamil causes and additive inhibitory effect as if verapamil is applied alone. By increasing the external calcium concentration from 10-4 M to 10-3 M the optimum of positive phototaxis is shifted to higher fluence rates. This shifting occurs also in the presence of channel blockers, but the strength of the positive reaction is influenced. These results point to the involvement of calcium channels in both phototaxis and motility, but simultaneously demonstrate the different sensitivity of the two processes to these drugs.

Key words

Chlamydomonas reinhardtii Phototaxis Motility Flagellates Flagellar movement Calcium Calcium channel blockers 

Abbreviations

DIL

diltiazem (=benzothiazepine)

FLU

flunarizine (=(E)-1-(bis-(4-fluorophenyl(methyl)-4-(3-phenyl-2-propenyl)piperazinex2HCl)

NIM

nimodipine (=1,4-dihydropyridine)

VER

verapamil (=diphenylalkylamine) CaM, calmodulin

PDE

phosphodiesterase

DMSO

dimethylsulfoxide

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References

  1. Collis PS, Weeks DP (1978) Selective inhibition of tubulin synthesis by amiprophosmethyl during flagellar regeneration in Chlamydomonas reinhardtii. Science 202:440Google Scholar
  2. Diehn B, Feinleib M, Haupt W, Hildebrand E, Lenci F, Nultsch W (1977) Terminology of behavioral responses of motile microorganisms. Photochem Photobiol 26:559–560Google Scholar
  3. Enyeart JJ, Hinkle PM (1984) The calcium agonist Bay K 8644 stimulates secretion from a pituitary cell line. Biochem Biophys Res Commun 122:991–996Google Scholar
  4. Gitelman SE, Witman GB (1980) Purification of calmodulin from Chlamydomonas: calmodulin occurs in cell bodies and flagella. J Cell Biol 87:764–770Google Scholar
  5. Glossmann H, Ferry DR, Goll A (1984) Molecular pharmacology of the calcium channel. In: Paton W, Mitchell J, Turner P (eds) Proceedings. Iuphar 9th International Congress of Pharmacology, London, pp 1–6Google Scholar
  6. Hess FD, Bayer DE (1977) Binding of the herbicide trifluralin to Chlamydomonas flagellar tubulin. J Sci Cell 24:351Google Scholar
  7. Kubo K, Matsuda Y, Kase H, Yamada K (1984) Inhibition of calmodulin-dependent cyclic nucleotide phosphodiesterase by flunarizine, a calcium entry blocker. Biochem Biophys Res Commun 124:315–321Google Scholar
  8. Nultsch W (1975) Phototaxis and photokinesis. In: Carlile MJ (ed) Primitive sensory and communication systems. The taxis and tropism of microorganisms and cells. Academic Press, London New York San Francisco, pp 29–90Google Scholar
  9. Nultsch W (1979) Effect of external factors on phototaxis of Chlamydomonas reinhardtii. III. Cations. Arch Microbiol 123:93–99Google Scholar
  10. Nultsch W (1983) The photocontrol of movement of Chlamydomonas. In: Cosens DJ, Vince-Prue D (eds) The biology of photoreception. Soc Exp Biol Symp XXXVI, pp 521–539Google Scholar
  11. Nultsch W, Throm G, v. Rimscha I (1971) Phototaktische Untersuchungen an Chlamydomonas reinhardtii Dangeard in homokontinuierlicher Kultur. Arch Microbiol 80:351–369Google Scholar
  12. Pfau J, Nultsch W, Rüffer U (1983) A fully automated and computerized system for simultaneous measurements of motility and phototaxis in Chlamydomonas. Arch Microbiol 135:259–264Google Scholar
  13. Schmidt JA, Eckert R (1976) Calcium couples flagellar reversal to photostimulation in Chlamydomonas reinhardtii. Nature (London) 262:713–715Google Scholar
  14. Senger HJ, Pfau J, Werthmüller K (1972) Continuous automatic cultivation of homocontinuous and synchronized microalgae. Methods Cell Physiol V:301–323Google Scholar
  15. Stavis RL (1974) The effect of azide on phototaxis in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 71:1824–1827Google Scholar
  16. Stavis RL, Hirschberg R (1973) Phototaxis in Chlamydomonas reinhardtii. J Cell Biol 59:367–377Google Scholar
  17. Triggle DJ (1981) Calcium antagonists: Basic chemical and pharmacological aspects. In: Weiss GB (ed) New perspectives on calcium antagonists. Am Physiol Soc 1981, Bethesda Maryland, pp 1–18Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Wilhelm Nultsch
    • 1
  • Jürgen Pfau
    • 1
  • Reiner Dolle
    • 1
  1. 1.Botanisches InstitutFachbereich Biologie der Philipps-Universität MarburgMarburg 1Federal Republic of Germany

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