Archives of Microbiology

, Volume 152, Issue 3, pp 273–279 | Cite as

Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species

  • W. Dangel
  • A. Tschech
  • G. Fuchs
Original Papers


The enzymes involved in the anaerobic degration of cyclohexanol were searched for in a denitrifying Pseudomonas species which metabolizes this alicyclic compound to CO2 anaerobically. All postulated enzyme activities were demonstrated in vitro with sufficient specific activities. Cyclohexanol dehydrogenase catalyzes the oxidation of the substrate to cyclohexanone. Cyclohexanone dehydrogenase oxidizes cyclohexanone to 2-cyclohexenone. 2-Cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase convert 2-cyclohexenone via 3-hydroxycyclohexanone into 1,3-cyclohexanedione. Finally, the dione is cleaved by 1,3-cyclohexanedione hydrolase into 5-oxocaproic acid. Some kinetic and regulatory properties of these enzymes were studied.

Key words

Alicyclic compounds Denitrification Cyclohexanol dehydrogenase Cyclohexanone dehydrogenase 2-Cyclohexenone hydratase 3-Hydroxycyclohexanone dehydrogenase 1,3-Cyclohexanedione hydrolase Phenol Aromatization 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • W. Dangel
    • 1
  • A. Tschech
    • 1
  • G. Fuchs
    • 1
  1. 1.Angewandte MikrobiologieUniversität UlmUlmFederal Republic of Germany

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