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Evidence for host resistance in 1,3 bis(2-chloroethyl)-1-nitrosourea treatment induced in syngeneic LSA lymphoma


The anticancer agent 1,3 Bis(2-chloroethyl)-1-Nitrosourea (BCNU) cures the advanced syngeneic LSA lymphoma of C57BL mice with high efficiency. The cured animals resist further tumor challenge by large numbers of viable syngeneic tumor cells. Growth assays of spleen proliferation of the intravenously inoculated tumor revealed a progressive-regressive pattern of spleen growth after LSA-tumor injection. Lymphoma colony forming units (LCFU) in the spleen initially increased then regressed. In vitro assays of serum showed a alack of cytotoxic activity in mice cured by BCNU. Added spleen, thymus, or bone-marrow cells were similarly ineffective. Spleen and bone-marrow cells from immune mice passively transferred to normal mice showed weak cytotoxic activity against the LSA tumor. BCNU-cured mouse cells were more effective in protection than those cured with Chlorozotocin (CLZ).

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Correspondence to Y. Maruyama.

Additional information

Supported by research grants nos. RO1 CA 13849, PO1 17786 from the National Cancer Institute, U.S. Public Health Service, U.S. Department of Health, Education and Welfare, Bethesda, Maryland 20014, The University of Kentucky Research Foundation, The Ephraim McDowell Cancer Network, and the Kircher Foundation

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Maruyama, Y., Williams, A., Feola, J.M. et al. Evidence for host resistance in 1,3 bis(2-chloroethyl)-1-nitrosourea treatment induced in syngeneic LSA lymphoma. J Cancer Res Clin Oncol 103, 107–118 (1982).

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Key words

  • Host resistance
  • Chemotherapy
  • Tumor regression
  • Induced resistance