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Effects of light intensity and nutrient availability on diel patterns of cell metabolism and growth in populations of Synechococcus spp.

Abstract

Between July 21 and August 8, 1984, phytoplankton were collected from the surface (2 m) and/or chlorophyll maximum of a neritic front, warm-core eddy 84-E and Wilkinson's Basin in the Northwest Atlantic Ocean and incubated up to 38 h in 200-liter vats. Effects of light intensity and nutrient availability on diel patterns of cell metabolism were analyzed in a 0.6- to 1-μm fraction, where Synechococcus spp. represented 80 to 100% of the total photoautotrophs. Populations held under in situ conditions exhibited daytime peaks in photosynthetic potential (Pmax) that were an order of magnitude higher than nighttime Pmax values. Daytime phasing of Pmax peaks had no relationship to asynchronous fluctuations in cellular activities of ribulose 1,5 bisphosphate carboxylase (RUBPCase) or phosphoenol pyruvate carboxylase (PEPCase), or to variations in chlorophyll content. Daytime Pmax peaks were about 12 h out of phase with nighttime maxima in the frequency of dividing cells (FDC). The phase relationship between Pmax and FDC could be altered by manipulating environmental conditions. High light exposure of depp populations did not affect timing of the Pmax peak, but its magnitude increased and coincided with increased RUBPCase activity and chlorophyll photobleaching. In the eddy population, a major shift in the timing of peak Pmax was induced when increased light intensity was accompanied by nutrient enrichment. This change coincided with major increases in cellular chlorophyll and carboxylating enzyme activity. Lowering irradiance and/or increasing nutrient availability elicited different diel pattern in cellular metabolism in surface populations from the eddy and from Wilkinson's Basin that appeared linked to differences in the nutrient status of the cells. Rates of cell division estimated from the percentage of dividing cells in preserved samples were 0.83 divisions d-1 in surface warm-core eddy populations, supporting the view that carbon and nitrogen turnover rates in oligotrophic waters can be sufficient to promote near optimal growth of Synechococcus spp.

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Literature cited

  1. Barlow, R. G. and R. S. Alberte: Photosynthetic characteristics of phycoerythrin-containing marine Synechococcus spp. Mar. Biol. 86, 63–74 (1985)

  2. Boczar, B. A., J. S. Gould, and B. B. Prézelin: Photoinhibition of photosynthesis, fluorescence, and chlorophyll a content in marine cyanobacteria. EOS Trans. Am. geophys. Union 52, 1085 (1983)

  3. Campbell, L.: Investigation of marine phycoerythrin-containing Synechococcus spp. (cyanobacteria) by immunofluorescence: distribution of different serogroups and growth rate measurements, 186 pp. Ph.D. thesis, SUNY Stony Brook 1985

  4. Campbell, L. and E. J. Carpenter: Diel patterns of cell division in marine Synechococcus spp. (Cyanobacteria): the use of the frequency of dividing cells technique to measure growth rate. Mar. Ecol. Prog. Ser. 32, 139–148 (1986)

  5. Carr, N. G. and B. A. Whitton (Eds.) The biology of blue-green algae. In: Botanical monographs, Vol. 9, 676 pp. Berkeley: University of California Press 1973

  6. Cuhel, R. L. and J. B. Waterbury: Biochemical composition and short term nutrient incorporation patterns in a unicellular marine cyanobacterium, Synechococcus (WH7803). Limnol. Oceanogr. 29, 370–374 (1984)

  7. Eppley, R. W.: Estimating phytoplankton growth rates in the central oligotrophic oceans. In: Primary productivity in the sea, pp 231–242. Ed. by P. G. Falkowski. New York: Plenum 1980

  8. Eppley, R. W.: Relations between nutrient assimilation and growth in phytoplankton with a brief review of estimates of growth rate in the ocean. In: Physiological bases of phytoplankton ecology. Ed. by T. Platt. Can. Bull. Fish. aquat. Sci., Ottawa 210, 251–263 (1981)

  9. Gieskes, W. W. C. and G. W. Kraay: Floristic and physiological differences between the shallow and the deep nanophytoplankton community in the euphotic zone of the open tropical Atlantic revealed by HPLC analysis of pigments. Mar. Biol. 91, 567–576 (1986)

  10. Glover, H. E.: The physiology and ecology of the marine cyanobacterial genus, Synechococcus, Vol. 3, pp 49–107. In: Advances in aquatic microbiology. Ed. by H. W. Jannasch and P. J. le B. Williams. New York: Academic Press 1985

  11. Glover, H. E., L. Campbell and B. B. Prézelin: Contribution of Synechococcus spp. to size-fractioned primary productivity in three water masses in the Northwest Atlantic. Mar. Biol. 91, 193–203 (1986)

  12. Glover, H. E. and I. Morris: Photosynthetic carboxylating enzymes in marine phytoplankton. Limnol. Oceanog. 24, 510–519 (1979)

  13. Glover, H. E. and I. Morris: Photosynthetic characteristics of coccoid marine cyanobacteria. Arch. Microbiol. 129, 42–46 (1981)

  14. Glover, H. E., D. A. Phinney and C. S. Yentsch: Photosynthetic characteristics of picoplankton compared with those of larger phytoplankton populations in various water masses in the Gulf of Maine. Biol. Oceanogr. 3, 223–248 (1985a)

  15. Glover, H. E., A. E. Smith and L. Shapiro: Diurnal variations in photosynthetic rates: comparisons of ultraphytoplankton with a larger phytoplankton size fraction. J. Plankt. Res. 7, 519–535 (1985b)

  16. Goldman, J. C.: Physiological processes, nutrient availability, and the concept of relative growth rate in marine phytoplankton ecology. In: Primary production in the sea, pp 175–199. Ed. by P. G. Falkowski. New York: Plenum Press 1980

  17. Jackson, G. A.: Phytoplankton growth and zooplankton grazing in oligotrophic oceans. Nature, Lond. 284, 439–441 (1980)

  18. Lewis, M. R. and J. C. Smith. A small volume, short-incubation time method for measurement of photosynthesis as a function of incident irradiance. Mar. Ecol. Prog. Ser. 13, 99–102 (1983)

  19. Li, W. W. K., D. V. Subba-Rao, W. G. Harrison, J. C. Smith, J. J. Cullen, B. Irwin and T. Platt: Autotrophic picoplankton in the tropical ocean. Science, Wash. D.C. 219, 292–295 (1983)

  20. McCarthy, J. J. and J. C. Goldman: Nitrogenous nitrition of marine phytoplankton in nutrient-depleted waters. Science, Wash. D.C. 203, 670–672 (1979)

  21. McDuff, R. E. and S. W. Chisholm: The calculation of in situ growth rates of phytoplankton populations from fractions of cells undergoing mitosis: a clarification. Limnol. Oceanogr. 27, 783–788 (1982)

  22. Ohki, K., J. G. Rueter and Y. Fujita: Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. theilbautii in synthetic medium. Mar. Biol. 91, 9–13 (1986)

  23. Platt, T., D. W. Subba Rao and B. Irwin: Photosynthesis of picoplankton in the oligotrophic ocean. Nature, Lond. 301, 702–704 (1983)

  24. Prézelin, B. B., M. Putt and H. E. Glover: Diurnal patterns in photosynthetic capacity and depth-dependent photosynthesisirradiance relationships in Synechococcus spp. and large phytoplankters in three water masses in the Northwest Atlantic. Mar. Biol. 91, 205–217 (1986a)

  25. Prézelin, B. B., G. Samuelsson and H. A. Matlick: Photosystem II photoinhibition and altered kinetics of photosynthesis during nutrient-dependent high-light photoadaptation in Gonyaulax polyedra. Mar. Biol. 93, 1–12 (1986b)

  26. Putt, M. and B. B. Prezelin: Observations of diel patterns of photosynthesis in cyanobacteria and nanoplankton in the Santa Barbara Channel during ‘el Nino.’ J. Plankt. Res. 7, 779–790 (1985)

  27. Smith, R. C., K. S. Baker and P. Dunstan: Fluorometric techniques for the measurement of ocean chlorophyll in the support of remote sensing. Ref. Rep. Scripps Inst. Oceanog. 81–17, 1–14 (1981)

  28. Sweeney, B. M.: Circadian timekeeping in eukaryotic cells; models and hypotheses. Prog. phycol. Res. 2, 189–225 (1983)

  29. Takahashi, M., K. Kikuchi and Y. Hara: Importance of picocyanobacteria biomass (unicellular, blue-green algae) in the phytoplankton population of the coastal waters off Japan. Mar. Biol. 89, 63–69 (1985)

  30. Waterbury, J. B., S. W. Watson and F. W. Valois: Preliminary assessment of the importance of Synechococcus spp. as oceanic primary producers. In: Primary production in the sea, pp 516–517. Ed. by P. G. Falkowski. New York: Plenum Press 1980

  31. Yentsch, C. S. and D. W. Menzel: A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence. Deep-Sea Res. 10, 443–448 (1963)

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Communicated by J. P. Grassle, Woods Hole

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Prézelin, B.B., Glover, H.E. & Campbell, L. Effects of light intensity and nutrient availability on diel patterns of cell metabolism and growth in populations of Synechococcus spp.. Mar. Biol. 95, 469–480 (1987). https://doi.org/10.1007/BF00409576

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Keywords

  • Chlorophyll
  • Phytoplankton
  • Synechococcus
  • Pyruvate Carboxylase
  • Phosphoenol Pyruvate Carboxylase