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Archiv für Mikrobiologie

, Volume 92, Issue 1, pp 69–83 | Cite as

A study of copulation, sporulation and meiotic segregation in Candida lipolytica

  • Claude M. Gaillardin
  • Véronique Charoy
  • Henri Heslot
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Summary

We have attempted to optimize conjugation and sporulation in Candida lipolytica, by studying the conditions of culture and growth.

Copulation between compatible strains is a rare event, particularly in the case of auxotrophic mutants. However, diploids can be selected for on minimal medium provided parents are suitable auxotrophs. These diploids can multiply vegetatively for many generations. They can also be induced to sporulate at a very high frequency.

Free ascospores were isolated by means of paraffin oil and segregations of markers could be studied. At first quite irregular, these segregations improved following a number of brother-sister matings. At the same time, the mean number of spores per ascus as well as spore germinability were considerably increased.

Keywords

Paraffin Candida Minimal Medium Ascus Rare Event 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Claude M. Gaillardin
    • 1
  • Véronique Charoy
    • 1
  • Henri Heslot
    • 1
  1. 1.Department of GeneticsInstitut National AgronomiqueParis

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