Archiv für Mikrobiologie

, Volume 55, Issue 1, pp 46–53 | Cite as

Cellular distribution of yeast pyruvate decarboxylase, and its induction by glucose

  • M. Ruiz-Amil
  • Ma. J. Fernández
  • L. Medrano
  • M. Losada
Article
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Summary

The cellular distribution of pyruvate decarboxylase and acetyl-CoA kinase in C. pulcherrima grown on glucose has been investigated. By using a mild procedure for the separation of the cytoplasmic and mitochondrial fractions, it could be demonstrated that both enzymes are almost exclusively localized in the cytoplasm.

The levels of pyruvate decarboxylase in Candida pulcherrima and Saccharomyces cheresiensis grown aerobically on different carbon sources have also been studied: it was high in cells from glucose, glucose plus acetate, or glucose plus pyruvate, and low in cells from acetate or pyruvate. By contrast, the content of acetyl-CoA kinase was always relatively constant. Evidence is also presented for the induction of pyruvate decarboxylase by glucose.

Keywords

Glucose Enzyme Carbon Source Pyruvate Candida 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Es wurde die Zellverteilung von Pyruvatdecarboxylase und Acetat-CoA-Kinase in mit Glucose gewachsenem Candida pulcherrima untersucht. Bei der Erhaltung der subcellularen Fraktionen, d. h. Cytoplasma und Mitochondrien, ist eine milde Methode angewandt worden. Es konnte gezeigt werden, daß Pyruvatdecarboxylase und Acetat-CoA-Kinase fast ausschließlich in der cytoplasmatischen Fraktion vorkommen.

Die Menge dieser Enzyme in mit verschiedenen Kohlenstoffquellen aerob gewachsenen Candida pulcherrima und Saccharomyces cheresiensis wurde ebenfalls untersucht. Die Ergebnisse zeigen zeigen einen hohen Pyruvat-decarboxylaseinhalt in Hefezellen aus Glucose, Glucose plus Acetat oder Glucose plus Pyruvat, im Gegensatz zu jenen aus Acetat oder Pyruvat, deren Inhalt in diesem Enzyme niedrig war. Die Werte für Acetat-CoA-Kinase zeigen aber keine deutlichen Änderungen. Außerdem wurde die Induktion von Pyruvatdecarboxylase durch Glucose nach-gewiesen.

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Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • M. Ruiz-Amil
    • 1
  • Ma. J. Fernández
    • 1
    • 2
  • L. Medrano
    • 1
  • M. Losada
    • 1
    • 3
  1. 1.Sección de Fisiología Celular, Instituto de Biología CelularC.S.I.C.MadridSpain
  2. 2.Dpto. Fermentaciones IndustrialesInstituto de QuímicaMadrid(Spanien)
  3. 3.Centro Investigaciones BiológicasInstituto de Biología Celular C.S.I.C.Madrid(Spanien)

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