Planta

, Volume 159, Issue 6, pp 570–578 | Cite as

Metabolism of phosphoenolpyruvate in the C4 cycle during photosynthesis in the phosphoenolpyruvate-carboxykinase C4 grass Spartina anglica Hubb.

  • Alison M. Smith
  • Harold W. Woolhouse
Article

Abstract

The aim of this work was to investigate the fate of phosphoenolpyruvate (PEP) produced by decarboxylation of oxaloacetate during photosynthesis in the bundle sheaths of leaves of the PEP-carboxykinase C4 grass Spartina anglica Hubb. Mesophyll protoplasts and bundle sheath cells were separated enzymically and used to investigate activities and distributions of putative enzymes of the C4 cycle and the photosynthetic carbon metabolism of bundle sheath cells. The results indicate that neither conversion of PEP to pyruvate nor its conversion to 3-phosphoglycerate can account for all of the carbon flux through the C4 cycle during photosynthesis. It is likely, therefore, either that PEP moves directly from bundle sheath to mesophyll or that more than one pathway of regeneration of PEP is involved in the C4 cycle in this plant.

Key words

Bundle sheath cell Mesophyll protoplast Phosphoenolpyruvate-carboxykinase C4 plant Phosphoenolpyruvate metabolism Photosynthesis (enzymes) Spartina 

Abbreviations

Chl

chlorophyll

PEP

phosphoenolpyruvate

Pi

phosphate

RuBP

ribulose-1,5-bisphosphate

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Alison M. Smith
    • 1
  • Harold W. Woolhouse
    • 1
  1. 1.ARC Research Group on PhotosynthesisJohn Innes InstituteNorwichUK

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