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Kompartimentbildungen in Hungerzellen von Saccharomyces cerevisiae

Formation of compartments in starved cells of Saccharomyces cerevisiae

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Summary

The influence of a 48 hour starvation on the fine structure of Saccharomyces cerevisiae was investigated. The most prominent structural changes were observed when cells from an exponentially growing culture were treated: Besides some already known particularities, the most conspicious features were more or less complex compartments and myelin-like bodies. Their localization and fine structure show some parallels to mesosomes, lomasomes as well as vacuoles.

The development of the various kinds of compartments is regarded as a consequence of a reversible loss of differentiation produced by the extreme starvation. Their physiological function is discussed in relation to the activated degradation processes, necessary for survival.

Zusammenfassung

Der Einfluß einer 48stündigen Aushungerung in Wasser auf die Feinstrucktur von Saccharomyces cerevisiae wird untersucht. Zellen aus der exponentiellen Vermehrungsphase zeigten danach die auffälligsten Veränderungen: Neben Strukturbesonderheiten bereits bekannter Art fallen mehr oder weniger komplexe Kompartimente und myelinartige Gebilde auf, die in ihrer Lokalisierung und Feinstruktur Parallelen zu Mesosomen, Lomasomen sowie Vacuolen aufweisen. Ihre Entstehung wird als Folge einer durch die extreme Aushungerung bedingten, reversiblen Entdifferenzierung gedeutet. Ihre Funktion wird im Zusammenhang mit der zum Überleben notwendigen Aktivierung von Abbauprozessen diskutiert.

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Literatur

  1. Beaton, C. D.: An electron microscope study of the mesosomes of a penicillinase-producing Staphylococcus. J. gen. Microbiol. 50, 37–42 (1968).

  2. Bladen, H. A., Nylen, M. U., Fitzgerald, R. J.: Internal structural of a Eubacterium sp. demonstrated by the negative staining technique. J. Bact. 88, 763–770 (1964).

  3. Bracker, C. E.: Ultrastructure of fungi. Ann. Rev. Phytopath. 5, 343–374 (1967).

  4. Branton, D., Southworth, D.: Fracture faces of frozen Chlorella and Saccharomyces cells. Exp. Cell Res. 47, 648–653 (1967).

  5. Calonge, F. D., Fielding, A. H., Byrde, R. J. W.: Multivesicular bodies in Sclerotinia fructigena and their possible relation to extracellular enzyme secretion. J. gen. Microbiol. 55, 177–184 (1969).

  6. Campbell, R.: Further electron microscope studies of the conidium of Alternaria brassicicola. Arch. Mikrobiol. 69, 60–68 (1969).

  7. Coulter, H. D.: Rapid and improved methods for embedding biological tissues in Epon 812 and Araldite 502. J. Ultrastruct. Res. 20, 346–355 (1967).

  8. Falk, H.: Lomasomen?. Ber. dtsch. bot. Ges. 82, 427–429 (1969).

  9. Gäb, J.: Rhythmische Bindung und Freisetzung von Glucose bei verschiedenen Hefestämmen. Diplomarbeit, T. U. Braunschweig 1970 (unveröffentlicht).

  10. Gezelius, K., Norkrans, B.: Ultrastructure of Debaryomyces hansenii. Arch. Mikrobiol. 70, 14–25 (1970).

  11. Ghosh, B. K., Sargent, M. G., Lampen, J. O.: Morphological phenomena associated with penicillinase induction and secretion in Bacillus licheniformis. J. Bact. 96, 1314–1328 (1968).

  12. Gottschalk, H. J.: Stoffwechselrhythmen bei aeroben Hefen. Dissertation, T. U. Braunschweig 1970 (unveröffentlicht).

  13. Günther, T., Kattner, W., Merker, H. J.: Über das Verhalten und die Lokalisation der sauren Phosphatase von Hefezellen bei Repression und Derepression. Exp. Cell. Res. 45, 133–147 (1967).

  14. Hashimoto, T., Yoshida, N.: Unique membranous system associated with glycogen synthesis in an imperfect fungus, Geotrichum candidum. 6th Internat. Congr. Electron Microscopy, Kyoto (1966). Vol. II: Biology, pp. 305–306. Tokyo: Maruzen Co. 1966.

  15. Kozar, F., Weijer, J.: Electron-dense structures in Neurospora crassa. I. Mesosomes. Canad. J. Genet. Cytol. 11, 613–616 (1969a).

  16. ——: Electron-dense structures in Neurospora crassa. II. Lomasome-like structures. Canad. J. Genet. Cytol. 11, 617–621 (1969b).

  17. Kraepelin, G.: Der Atmungsdefekt bei Hefezellen; eine kritische Betrachtung seiner Ursachen. I. Induktion und Reversibilität der „petite”-Mutation. Z. allg. Mikrobiol. 7, 287–325 (1967).

  18. Kraepelin, G.: Physiological rhythms in Saccharomyces cerevisiae populations: Respiratory deficiency, glucose uptake, excretion of nucleotide material. 5th meeting of the Federation of European Biochemical Societies. Czechoslovak Biochem. Soc. Abstract 259, Prag 1968.

  19. Kraepelin, G.: Physiological rhythms in petite mutation. antonie v. Leeuwenhoek 35, Suppl. Yeast Symposium, Den Haag 1969, Abstract C 13.

  20. Lewis, M. J., Phaff, H. J.: Release of nitrogenous substances by brewer's yeast. IV. Energetics in shock excretion of amino acids. J. Bact. 89, 960–966 (1965).

  21. Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).

  22. Manocha, M. S., Colvin, J. R.: Structure of the cell wall of Pythium debaryanum. J. Bact. 95, 1140–1152 (1968).

  23. Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963).

  24. Moore, R. T., McAlear, J. H.: Fine structure of mycota. 5. Lomasomes-previously uncharacterized hyphal structures. Mycologia (N.Y.) 53, 194–200 (1961).

  25. Nečas, O., Havelková, M.: Electron microscopy of the yeast protoplasts and their growing forms. Symp. Hefe-Protoplasten, Jena 1965, pp. 55–58. Berlin: Akademie Verlag 1967.

  26. Polakis, E. S., Bartley, W., Meek, G. A.: Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem. J. 90, 369–374 (1964).

  27. Remsen, C. C., Hess, W. M., Sassen, M. M. A.: Fine structure of germinating Penicillium megasporum conidia. Protoplasma (Wien) 64, 439–451 (1967).

  28. Reynolds, E. S.: The use of led citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).

  29. Rüger, H. J.: Gärungsabhängige Vermehrungsrhythmen bei verschiedenen Hefetypen. Untersuchungen an flaktionierten Populationen. Dissertation, T. U. Braunschweig 1967 (unveröffentlicht).

  30. Wallace, P. G., Huang, M., Linnane, A. W.: The biogenesis of mitochondria. II. The influence of medium composition on the cytology of anaerobically grown Saccharomyces cerevisiae. J. Cell Biol. 37, 207–220 (1968).

  31. Weimberg, R., Orton, W. L.: Elution of exocellular enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae. J. Bact. 91, 1–13 (1966).

  32. Wells, K.: Ultrastructural features of developing and mature basidia and basidiospores of Schizophyllum commune. Mycologia (N.Y.) 57, 236–261 (1965).

  33. Zachariah, K., Fitz-James, P. C.: The structure of phialides in Penicillium claviforme. Canad. J. Microbiol. 13, 249–256 (1967).

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Hinkelmann, W., Kraepelin, G. Kompartimentbildungen in Hungerzellen von Saccharomyces cerevisiae . Archiv. Mikrobiol. 74, 258–272 (1970). https://doi.org/10.1007/BF00408886

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