By indirect immunofluorescence and preembedding peroxidase-diaminobenzidine technique the localization of polyclonal and monoclonal antibodies against α1, α2 and α3 isoforms of the Na,K-ATPase were studied in rat myocardium.
The α1-subunit was identified predominantly on sarcolemma of cultured myocytes, neonatal, as well as adult cardiocytes. The α2 signal was localized around nuclei of cultured cardiocytes, very weak signals were seen in neonatal and more intense signal, were dispersed throughout the adult myocytes. The α3-subunit immunoreactivity was weak and localized in cell processes connecting individual cultured cells, on sarcolemma and intercalated discs of neonatal cells and very weak in adult working myocytes. Cytochemically demonstrated ouabain resistant Na,K-ATPase localized in junctional sarcoplasmic reticulum may represent α1 isoenzyme which is directly involved in modulation of action potential fluxes.
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Slezak, J., Schulze, W., Stefankova, Z. et al. Localization of α1,2,3-subunit isoforms of Na,K-ATPase in cultured neonatal and adult rat myocardium: The immunofluorescence and immunocytochemical study. Mol Cell Biochem 163, 39–45 (1996). https://doi.org/10.1007/BF00408639
- immunoelectron microscopy