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Archiv für Mikrobiologie

, Volume 60, Issue 4, pp 303–313 | Cite as

The fine structure of akinete formation and germination in Cylindrospermum

  • Marcia Madsen Miller
  • Norma J. Lang
Article

Summary

Akinete formation and germination were studied in a species of Cylindrospermum using the electron microscope. The differentiation of a vegetative cell into an akinete is characterized by cell enlargement, sheath condensation, deposition of several spore envelope layers, including a dense fibrillar layer and deposition of large cyanophycin granules. The mature akinete in addition to the multilayered envelope retains internally a large number of cyanophycin granules, a photosynthetic thylakoid system, polyhedral bodies, lipid deposits and nucleoplasmic regions. Germination of the akinete can take place in several modes differing in detail. Most frequently the spore envelope remains intact and the germling which may or may not have divided emerges through a pore at one end of the envelope. The photosynthetic thylakoid system appears to increase by the fusion of small vesicles found in the cytoplasm. Alpha-granules are numerous and cyanophycin is nearly absent in the germling.

Keywords

Lipid Electron Microscope Vegetative Cell Fine Structure Cell Enlargement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bowen, C. C.: The organization of the Cyanophycean cell. 10th Int. Bot. Congr. (Edinburgh), Proc., p. 215 (1964).Google Scholar
  2. Chapman, J. A., and M. R. J. Salton: A study of several blue-green algae in the electron microscope. Arch. Mikrobiol. 44, 311–322 (1962).PubMedGoogle Scholar
  3. Echlin, P., and L. Morris: The relationship between blue-green algae and bacteria. Biol. Rev. 40, 143–187 (1965).PubMedGoogle Scholar
  4. Fogg, G. E.: Growth and heterocyst production in Anabaena cylindrica Lewin. III. The cytology of heterocysts. Ann. Bot. Lond., N. S. 15, 23–36 (1951).Google Scholar
  5. Fritsch, F. E.: The structure and reproduction of the algae. Vol. II, pp. 768–898. Cambridge: Cambridge University Press 1945.Google Scholar
  6. Fuhs, G. W.: Cytochemisch-elektronenmikroskopische Lokalisierung der Ribonucleinsäure und des Assimilats in Cyanophyceen. Protoplasma (Wien) 56, 178–187 (1963).Google Scholar
  7. Gerloff, G. C., G. P. Fitzgerald, and F. Skoog: The isolation, purification and culture of blue-green algae. Amer. J. Bot. 37, 216–218 (1950).Google Scholar
  8. Giesy, R. M.: A light and electron microscope study of interlamellar polyglucoside bodies in Oscillatoria chalybia. Amer. J. Bot. 51, 388–396 (1964).Google Scholar
  9. Glade, R.: Zur Kenntnis der Gattung Cylindrospermum. Beitr. Biol. Pflanz. 12, 295–346 (1914).Google Scholar
  10. Harder, R.: Über die Beziehung des Lichtes zur Keimung von Cyanophyceen Sporen. Jb. wiss. Bot. 58, 237–294 (1917).Google Scholar
  11. Jost, M.: Die Ultrastruktur von Oscillatoria rubescens D. C. Arch. Mikrobiol. 50, 211–245 (1965).Google Scholar
  12. Leak, L. V., and G. B. Wilson: Electron microscopic observations on a blue-green alga, Anabaena sp. Canad. J. Genet. Cytol. 7, 237–249 (1965).Google Scholar
  13. Madsen, M. J.: Electron microscopy of the blue-green algae, Cylindrospermum and Gloeotrichia. M. A. Thesis. University of California, Davis 1966.Google Scholar
  14. Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1964).PubMedGoogle Scholar
  15. Pankratz, H. S., and C. C. Bowen: Cytology of blue-green algae. I. The cells of Symploca muscorum. Amer. J. Bot. 50, 387–399 (1963).Google Scholar
  16. Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).CrossRefPubMedGoogle Scholar
  17. Ris, H., and R. N. Singh: Electron microscope studies on blue-green algae. J. biophys. biochem. Cytol. 9, 63–80 (1961).PubMedGoogle Scholar
  18. Wildon, D. C., and F. V. Mercer: The ultrastructure of the heterocyst and akinete of the blue-green algae. Arch. Mikrobiol. 47, 19–31 (1963).Google Scholar
  19. Wolk, C. P.: Heterocyst germination under defined conditions. Nature (Lond.) 205, 201–202 (1965a).Google Scholar
  20. —: Control of sporulation in a blue-green alga. Devt. Biol. 12, 15–35 (1965b).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Marcia Madsen Miller
    • 1
  • Norma J. Lang
    • 1
  1. 1.Botany DepartmentUniversity of CaliforniaDavis

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