Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Zur Charakterisierung der Pectinase von Coniophora cerebella Duby

  • 26 Accesses

  • 6 Citations


  1. 1.

    6 investigated strains of Coniophora cerebella proved to be very uniform in growth and rate of enzyme synthesis.

  2. 2.

    Coniophora cerebella secretes a pectinase in high activity into the culture fluid when galacturonic acid, pectic acid or pectin are present.

  3. 3.

    The activity of the extracellular enzyme is higher than of the intracellular one. No differences of the properties could be found.

  4. 4.

    Pectin-methylesterase (pectase) activity is negligible or absent.

  5. 5.

    The activity of the pectinase is dependent on the methoxy-content of the pectin and of its origin. Low esterified pectin is faster attacked than high esterified pectin. Therefore the enzyme ist to be classified as an Endo-polygalacturonase.

  6. 6.

    The optimal pH-value for pectinase and pectase is 5.0. At 51° C the pectinase is inactivated for 50% after 5 min incubation.

  7. 7.

    Whether an Exo-Polygalacturonase (saccharifying enzyme) is produced, could not be established.

This is a preview of subscription content, log in to check access.


  1. Albersheim, P., H. Neukom u. H. Deuel: Über die Bildung von ungesättigten Abbauprodukten durch ein pectinabbauendes Enzym. Helv. chim. Acta 43, 1422 (1960).

  2. Aschan, K., and B. Norkrans: A study in the cellulolytic variation for wildtypes and mutants of Collybia velutipes. Physiol. Plantarum (Copenh.) 6, 564–583 (1953).

  3. Demain, A. L., and H. J. Phaff: Composition and action of yeast polygalacturonase. Nature (Lond.) 174, 515 (1954).

  4. ——: Recent advances in the enzymatic hydrolysis of pectic substances. Wallenstein Laboratories Communications 20, 119 (1957).

  5. Deuel, H., and E. Stutz: Pectic substances and pectic enzymes. Advanc. Enzymol. 20, 341 (1958).

  6. Endo, A.: Studies on pectolytic enzymes of molds. Part. III. General characteristics of pectolytic enzymes produced by Coniothyrium diplodiella. Agr. Biol. Chem. 25, 394 (1961).

  7. Fåhraeus, G., V. Tullander and H. Ljunggren: Production of high laccase yields in cultures of fungi. Physiol. Plantarum (Copenh.) 11, 631 (1958).

  8. Gaponenkow, G. K., u. S. I. Prozenko Über Spaltung der Pektinstoffe durch Fermente von Mikroorganismen und die chemische Natur der Endprodukte. Mikrobiologija 29, 658 (1960) (russisch).

  9. Henglein, E. A.: Die Uron- und Polyuronsäuren (Pektin und Alginsäure). Handbuch d. Pflanzenphysiol. Bd. 6, S. 405. Berlin, Göttingen, Heidelberg: Springer 1958.

  10. Keilin, D., and E. F. Hartree: Purification of horseradish-peroxidase and comparison of its properties with those of catalase and methaemoglobin. Biochem. J. 49, 88 (1951).

  11. Kertesz, J. I.: Pectic enzymes. Methods in Enzymology, Vol. 1, p. 158. New York: Academic Press Inc. 1955.

  12. Lyr, H.: Die Bildung von Ektoenzymen durch holzzerstörende und holzbewohnende Pilze auf verschiedenen Nährböden. Arch. Mikrobiol. 33, 266 (1959).

  13. —: Hemmungsanalytische Untersuchungen an einigen Ektoenzymen holzzerstörender Pilze. Enzymologia 22, 231 (1961).

  14. —: Über das Vorkommen von Mannanase bei Pilzen. Z. allg. Mikrobiol. 3, 25 (1963).

  15. —, u. H. Ziegler: Enzymausscheidung und Holzabbau durch Phellinus igniarius Quél. und Collybia velutipes Curt. Phytopath. Z. 35, 173 (1959).

  16. Mill, P. J., and R. Tuttobello: The Pectic Enzymes of Aspergillus niger. 2. Endopolygalacturonase. Biochem. J. 79, 57 (1961).

  17. Nagel, Ch. W., and R. H. Vaughn: Comparison of growth and pectolytic enzyme production by Bacillus polymyxa. J. Bact. 83, 1 (1962).

  18. Patel, D. S., and H. J. Phaff. Studies on the purification of tomato polygalacturonase. Food Res. 25, 37 (1960).

  19. Phaff, H. J.: The production of exocellular pectic enzymes by Penicillium chrysogenum. I. On the formation and adaptive nature of polygalacturonase and pectinesterase. Arch. Biochem. 13, 67–81 (1947).

  20. Roboz, E., W. Barrett and E. L. Tatum: Breakdown of pectic substances by a new enzyme from Neurospora. J. biol. Chem. 195, 459 (1952).

  21. Thomas, R.: Fungal cellulases VII. Stachybotrys atra. Production and properties of the cellulolytic enzyme. Aust. J. biol. Sci. 9, 159 (1956).

  22. Tomes, M. P., K. B. Cooke, F. Souter and N. F. Macclagan: Spectrophotometric determination of serum proteins in the region 200–220 mμ. Proteides of the Biological Fluids, p. 37. Amsterdam: Elsevier Publ. Comp. 1960.

  23. Uritani, I., and M. A. Stahmann: Pectolytic enzymes of Ceratocystis fimbriata. Phytopath. J. 51, 277 (1961).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lyr, H. Zur Charakterisierung der Pectinase von Coniophora cerebella Duby. Archiv. Mikrobiol. 45, 198–209 (1963).

Download citation