Advertisement

Archives of Microbiology

, Volume 139, Issue 4, pp 332–337 | Cite as

Functional relationship between protein-bound and free factor F430 in Methanobacterium

  • Dorothe Ankel-Fuchs
  • Rolf Jaenchen
  • Norbert A. Gebhardt
  • Rudolf K. Thauer
Original Papers

Abstract

Factor F430 is a nickel porphinoid present in all methanogenic bacteria. It is a component of methyl-CoM reductase to which it is tightly but not covalently bound. Evidence is presented that in Methanobacterium thermoautotrophicum grown on nickel sufficient medium only approximately 30% of total F430 is associated with methyl-CoM reductase and that 70% is present in a non-bound, free form. When such cells were transferred to a nickel deficient medium the bacteria continued to grow although synthesis of total F430 stopped. During growth in the absence of nickel the amount of total F430 per 1 culture remained constant and that per g cells decreased. The ratio of free F430 to bound F430, however, changed. Free F430 was converted into the protein-bound form until almost all of the free F430 had disappeared. The kinetics of labelling with 63Ni of free and bound F430 agreed rather well with that calculated for a precursor-product relationship between free and bound F430.

Key words

Factor F430 Coenzyme F430 Nickel porphinoid Tetrapyrroles Methyl-CoM reductase Methanogenic bacteria Methanobacterium thermoautotrophicum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ankel-Fuchs D (1983) Eigenschaften und Funktionen von freiem und proteingebundenem Faktor F430 in Methanobacterium thermoautotrophicum. Diploma thesis, University of MarburgGoogle Scholar
  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  3. Brandis A, Thauer RK, Stetter KO (1981) Relatedness of strains ΔH and Marburg of Methanobacterium thermoautotrophicum. Zbl Bakt Hyg I Abt Orig C 2:311–317Google Scholar
  4. Diekert G, Klee B, Thauer RK (1980a) Nickel, a component of factor F430 from Methanobacterium thermoautotrophicum. Arch Microbiol 124:103–106PubMedGoogle Scholar
  5. Diekert G, Weber B, Thauer RK (1980b) Nickel dependence of factor F430 content in Methanobacterium thermoautotrophicum. Arch Microbiol 127:273–278Google Scholar
  6. Diekert G, Gilles HH, Jaenchen R, Thauer RK (1980c) Incorporation of 8 succinate per mol nickel into factors F430 by Methanobacterium thermoautotrophicum. Arch Microbiol 128:256–262PubMedGoogle Scholar
  7. Diekert G, Jaenchen R, Thauer RK (1980d) Biosynthetic evidence for a nickel tetrapyrrole structure of factor F430 from Methanobacterium thermoautotrophicum. FEBS Lett 119:118–120CrossRefPubMedGoogle Scholar
  8. Diekert G, Konheiser U, Piechulla K, Thauer RK (1981) Nickel requirement and factor F430 content of methanogenic bacteria. J Bacteriol 148:459–464PubMedGoogle Scholar
  9. Ellefson WL, Whitman WB, Wolfe RS (1982) Nickel-containing factor F430: Chromophore of the methylreductase of Methanobacterium. Proc Natl Acad Sci 79:3707–3710PubMedGoogle Scholar
  10. Gilles H, Thauer RK (1983) Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel-containing factor F430 in Methanobacterium thermoautotrophicum. Eur J Biochem 135:109–112PubMedGoogle Scholar
  11. Gilles H, Jaenchen R, Thauer RK (1983) Biosynthesis of 5-aminolevulinic acid in Methanobacterium thermoautotrophicum. Arch Microbiol 135:237–240Google Scholar
  12. Gunsalus RP, Wolfe RS (1978) Chromophoric factors F342 and F430 of Methanobacterium thermoautotrophicum. FEMS Microbiol Lett 3:191–193CrossRefGoogle Scholar
  13. Hausinger RP, Orme-Johnson WH, Walsh C (1984) The nickel tetrapyrrole cofactor F430: Comparison of the forms bound to methyl-S-coenzyme M reductase and protein-free in cells of Methanobacterium thermoautotrophicum ΔH. Biochemistry, 23:801–804Google Scholar
  14. Jaenchen R, Diekert G, Thauer RK (1981) Incorporation of methionine-derived methyl groups into factor F430 by Methanobacterium thermoautotrophicum. FEBS Lett 130:133–136CrossRefGoogle Scholar
  15. Keltjens JT (1982) Coenzyme of methanogenesis. Doctoral thesis of the University of Nijmegen, The NetherlandsGoogle Scholar
  16. Keltjens JT, Whitman WB, Caerteling CG, van Kooten AM, Wolfe RS, Vogels GD (1982) Presence of coenzyme M in the prosthetic group (coenzyme MF430) of methylcoenzyme M reductase from Methanobacterium thermoautotrophicum. Biochem Biophys Res Commun 108:495–503PubMedGoogle Scholar
  17. Keltjens JT, Caerteling CG, van Kooten AM, van Dijk HF, Vogels GD (1983a) Chromophoric derivatives of coenzym MF430, a proposed coenzyme of methanogenesis in Methanobacterium thermoautrophicum. Arch Biochem Biophys 223:235–253PubMedGoogle Scholar
  18. Keltjens JT, Caerteling CG, van Kooten AM, van Dijk HF, Vogels GD (1983b) 6,7-Dimeethyl-8-ribityl-5,6,7,8-tetrahydrolumazine, a proposed constituent of coenzyme MF430 from methanogenic bacteria. Biochim Biophys Acta 743:351–358Google Scholar
  19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680–685Google Scholar
  20. Livingston DA, Pfaltz A, Schreiber J, Eschenmoser A, Ankel-Fuchs D, Moll J, Jaenchen R, Thauer RK (1984) Zur Kenntnis des Faktors F430 aus methanogenen Bakterien: Struktur des proteinfreien Faktors. Helv Chim Acta 67:334–351Google Scholar
  21. Moura J, Moura JJG, Santos H, Xavier AV, Burch G, Peck HD Jr, Le Gall J (1983) Proteins containing the factor F430 from Methanosarcina barkeri and Methanobacterium thermoautotrophicum — isolation and properties. Biochim Biophys Acta 742:84–90Google Scholar
  22. Pfaltz A, Jaun B, Fässler A, Eschenmoser A, Jaenchen R, Gilles HH, Diekert G, Thauer RK (1982) Zur Kenntnis des Faktors F430 aus methanogenen Bakterien: Struktur des porphinoiden Ligandsystems. Helv Chim Acta 65:828–865Google Scholar
  23. Schönheit P, Moll J, Thauer RK (1980) Growth parameters (K s, μ max, Y s) of Methanobacterium thermoautotrophicum. Arch Microbiol 127:59–65Google Scholar
  24. Whitman WB, Wolfe RS (1980) Presence of nickel in factor F430 from Methanobacterium bryantii. Biochem Biophys Res Commun 92:1196–1201PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Dorothe Ankel-Fuchs
    • 1
  • Rolf Jaenchen
    • 1
  • Norbert A. Gebhardt
    • 1
  • Rudolf K. Thauer
    • 1
  1. 1.Fachbereich Biologie, MikrobiologiePhilipps-UniversitätMarburgFederal Republic of Germany

Personalised recommendations