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Archives of Microbiology

, Volume 150, Issue 4, pp 394–399 | Cite as

Evidence for urea cycle activity in Sporosarcina ureae

  • Stephen E. Gruninger
  • Manuel Goldman
Original Papers

Abstract

Sporosarcina ureae BS 860, a motile, sporeforming coccus, possesses the enzymes required for a functioning urea (ornithine) cycle. This is only the second known example of urea cycle activity in a prokaryote. Specific activities are reported for ornithine carbamoyltransferase, argininosuccinase, arginase, and urease. Although argininosuccinate synthetase activity could not be detected directly in crude cell extracts, indirect evidence from radiocarbon tracing data for arginine synthesis from the substrate, l-[1-14C]-ornithine, strongly suggest the presence of this or other similar enzyme activity. Furthermore, good growth in defined media containing either 1.0% glutamine, ornithine, or citrulline as sole carbon sources suggests argininosuccinate synthetase activity is necessary for arginine synthesis. The effect of varying pH on arginase and urease activities indicate that these two enzymes may function within the context of the urea cycle to generate ammonia for amino acid synthesis, as well as for raising the pH of the growth micro-environment.

Key words

Sporosarcina ureae Urea cycle Ornithine cycle Arginase Urease 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Stephen E. Gruninger
    • 1
  • Manuel Goldman
    • 1
  1. 1.Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA

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