Archiv für Mikrobiologie

, Volume 42, Issue 2, pp 138–153 | Cite as

The growth of Spirillum volutans ehrenberg in mixed and pure cultures

  • Beatrice T. Rittenberg
  • Sydney C. Rittenberg


The growth of Spirillum volutans in mixed culture was studied and liquid media were developed in which it grew rapidly and attained populations of over a million cells per ml. By taking advantage of their rapid and unidirectional motility, spirilla were separated from the mixed population by migration in capillary tubes. Pure cultures were achieved by growing the separated spirilla inside of dialysis sacks in contact with mixed cultures on the outside, and also by growth in an asparagine-mineral salts medium supplemented with an extract of Escherichia coli.


Escherichia Coli Migration Liquid Medium Pure Culture Mixed Culture 
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Copyright information

© Springer-Verlag 1962

Authors and Affiliations

  • Beatrice T. Rittenberg
    • 1
    • 2
  • Sydney C. Rittenberg
    • 1
    • 2
  1. 1.Hopkins Marine StationStanford UniversityPacific Grove
  2. 2.the Department of BacteriologyUniversity of Southern CaliforniaLos Angeles

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