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Physiological studies on Phymatotrichum omnivorum

II. Physiocochemical properties of glycogen

Summary

The physical and chemical properties and molecular structure of glycogen from Phymatotrichum omnivorum (Shear) Dugg. were studied. Glycogen samples from rabbit liver and shellfish were compared with Phymatotrichum glycogen in physicochemical properties. Analyses of purity showed the isolated glycogen was highly pure. Purified glycogen had an optical rotation of + 183.3°, and optical rotatory dispersion showed the absence of optically active substances between 200 and 600 nm. Maximum absorption of the glycogen-iodine complex was at 395 nm. I.R. spectra confirmed the α-1,4 linkage as well as α-d-glucopyranose units of the glycogen molecule. Alpha- and Beta-amylase studies indicated the average chain length was 13 glucose units with 43% limit dextrin. Results of periodate oxidation of glycogen agreed closely with the result of the amylase studies.

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References

  1. Betz, N. L., Nettles, W. C., Novak, A. F.: Physiocochemical characteristics of glycogen from Anthonomus grandis. Comp. Biochem. Physiol. 24, 163–175 (1968).

  2. Carrol, N. V., Longley, R. W., Roe, J. H.: The determination of glycogen in liver and muscle by use of the anthrone reagent. J. biol. Chem. 220, 583–593 (1956).

  3. Djerassi, C.: Optical rotatory disperison. New York: McGraw-Hill Book Co. 1960.

  4. Dunlap, A. A.: A convenient soil culture method for obtaining sclerotia of the cotton root rot fungus. Amer. J. Bot. 28, 945–947 (1941).

  5. Ergle, D. R.: The glycogen content of Phymatotrichum sclerotia. J. Amer. chem. Soc. 69, 2061–2062 (1947).

  6. Fales, F. W.: A reproducible periodate oxidation method for the determination of glycogen end-groups. Analyt. Chem. 31, 1898–1900 (1959).

  7. Folch, J. M., Lees, M., Sloane-Stanley, G. H.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957).

  8. Harris, G., MacWilliam, I. C.: A dipping technique for revealing sugars on paper chromatograms. Chem. Int. (Lond.) 73, 249 (1954).

  9. Kjolberg, O. G., Manners, D. J., Wright, A.: α-1,4 glucosans. XVIII. The molecular structure of some glycogens. Comp. Biochem. Physiol. 8, 353–365 (1963).

  10. Manners, D. J.: The molecular structure of glycogens. Advanc. Carbohyd. Chem. 12, 261–298 (1957).

  11. Manners, D. J., Wright, A.: Determination of the average chain length of glycogens by α-amylolysis. J. chem. Soc. 1962, 1597–1602.

  12. Moore, S., Stein, W. H.: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. biol. Chem. 211, 907–913 (1954).

  13. Northcote, D. H.: The molecular structure and shape of yeast glycogen. Biochem. J. 53, 348–352 (1953).

  14. Rogers, C. H.: Cotton root rot studies with special reference to sclerotia, cover crops, rotation tillage, seedling rates, soil fungicides and effects on seed quality. Tex. Agr. exp. Sta. Bull. 614 (1942).

  15. Somogyi, M.: Notes on sugar determination. J. biol. Chem. 195, 19–23 (1952).

  16. Sumi, M.: Chemical constituents of spores of Aspergillus oryzae. Biochem. Z. 195, 161–174 (1928).

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Gunasekaran, M. Physiological studies on Phymatotrichum omnivorum . Archiv. Mikrobiol. 84, 69–76 (1972). https://doi.org/10.1007/BF00408083

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Keywords

  • Glucose
  • Chain Length
  • Physicochemical Property
  • Amylase
  • Active Substance