The physical and chemical properties and molecular structure of glycogen from Phymatotrichum omnivorum (Shear) Dugg. were studied. Glycogen samples from rabbit liver and shellfish were compared with Phymatotrichum glycogen in physicochemical properties. Analyses of purity showed the isolated glycogen was highly pure. Purified glycogen had an optical rotation of + 183.3°, and optical rotatory dispersion showed the absence of optically active substances between 200 and 600 nm. Maximum absorption of the glycogen-iodine complex was at 395 nm. I.R. spectra confirmed the α-1,4 linkage as well as α-d-glucopyranose units of the glycogen molecule. Alpha- and Beta-amylase studies indicated the average chain length was 13 glucose units with 43% limit dextrin. Results of periodate oxidation of glycogen agreed closely with the result of the amylase studies.
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Gunasekaran, M. Physiological studies on Phymatotrichum omnivorum . Archiv. Mikrobiol. 84, 69–76 (1972). https://doi.org/10.1007/BF00408083
- Chain Length
- Physicochemical Property
- Active Substance