Archives of Microbiology

, Volume 141, Issue 3, pp 225–228 | Cite as

Aromatic metabolism in Rhizobium trifolii-catechol 1,2-dioxygenase

  • Y. P. Chen
  • A. R. Glenn
  • M. J. Dilworth
Original Papers


Catechol 1,2-dioxygenase has been purified 46-fold from cells of Rhizobium trifolii TA1 grown on benzoate plus glucose. The dioxygenase had a molecular weight of 107,000 and a sub-unit molecular weight of 59,000. The enzyme had a Km of 2 μM for catechol and also cleaved 4-methylcatechol. The dioxygenase contained 2 g atoms of Fe3+ per mole of enzyme which could be removed by treatment with 1,10-phenanthroline, resulting in a complete loss of activity; reactivation of the enzyme occurred specifically with Fe3+.

Key words

Rhizobium trifolii Aromatic metabolism in catechol 1,2-dioxygenase R. trifolii 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Y. P. Chen
    • 1
  • A. R. Glenn
    • 1
  • M. J. Dilworth
    • 1
  1. 1.Nitrogen Fixation Research Group, School of Environmental and Life SciencesMurdoch UniversityMurdoch

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