Studies on the substrate range of Clostridium kluyveri; the use of propanol and succinate
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The metabolism of Clostridium kluyveri has been extensively studied, but the range of substrates C. kluyveri can use for growth has not been fully explored. The use of propanol and succinate as growth substrates were established. C. kluyveri grows on acetate with propanol replacing ethanol. The principle carbon containing products were propionate, valerate, butyrate and hexanoate with traces of heptanoate. When grown with ethanol and succinate the principle carbon-containing products were acetate, butyrate and hexanoate. Hexanol was found as a product when incubated with ethanol and succinate 4-hydroxybutyrate or 3-butenoate. 5-Hexenoate was also a product of 3-butenoate and ethanol metabolism. The splitting of succinate into 2 acetates was indicated with ethanol providing the necessary reducing equivalents. Hydrogen was also found as a source of reducing equivalents but could not replace ethanol. A mechanism of succinate metabolism to acetate was proposed which accounts for growth yield, energetics considerations, carbon balances, production of side products and intermediates.
Key wordsSuccinate Propanol Hexanoate Anaerobic Metabolism Fermentation
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