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Chemical analysis of the wall of the yeast form of Taphrina deformans

Abstract

Glucose is the main component of the cell wall of the yeast form of Taphrina deformans. On the other hand the glucosamine content is very low (less than 1%). Other sugars are mannose, galactose and rhamnose; their content depends on the age of the cultures. The protein content is less than 5% and lipids less than 2%. Most of the isolated cell wall is alkalisoluble: the insoluble fraction is less than 8% of the total wall. Three polysaccharides have been detected: an α-glucan containing mostly α(1→3) and perhaps a few α(1→4) glycosidic linkages, a β-glucan containing β(1→3) and β(1→6) linkages, and a heteropolysaccharide fraction containing mannose, galactose and rhamnose. Chitin, if present, forms no more than 2‰ of the total wall. The biochemical characteristics of the cell wall of the yeast form of T. deformans distinguish it from that of filamentous Ascomycetes and Basidiomycetes and also of most of the yeasts. It is with the Schizosaccharomyces wall that they have the most features in common.

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References

  1. Anderson RJ (1939) cited by Asselineau J (1962) Les lipides bactériens. Herman, Paris

  2. Arx JA von, Walt JP van der, Liebenberg NVDM (1982) The classification of Taphrina and other fungi with yeast-like cultural states. Mycologia 74:285–286

  3. Auriol P, Touzé A (1970) Sur les polyosides des parois cellulaires d'organismes végétaux sains et parasités. Physiol Vég 8:263–275

  4. Bacon JSD, Jones D, Farmer VC, Weebley DM (1968) The occurrence of α(1→3) glucan in Cryptococcus, Schizosaccharomyces and Polyporus species and its hydrolysis by a Streptomyces culture filtrate lysing cell walls of Cryptococcus. Biochim Biophys Acta 158:313–315

  5. Barker SA, Bourne EJ, Stacey M, Whiffen DH (1954) Infrared spectra of carbohydrates. I. Some derivatieves of d-glucopyranose. J Chem Soc :171–176

  6. Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468

  7. Boas NF (1953) Method for determination of hexosamines in tissues. J Biol Chem 204:553–563

  8. Bush DA, Horisberger M, Horman C, Wursch P (1974) The wall structure of Schizosaccharomyces pombe. J Gen Microbiol 81: 199–206

  9. Deshusses J, Berthoud S, Porternak TH (1969) Recherches biochimiques sur Schizosaccharomyces pombe en fonction des conditions de culture et de l'action d'inhibiteurs. II. Composition des parois cellulaires. Biochim Biophys Acta 176:803–812

  10. Dubois NK, Gilles K, Hamilton JL, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

  11. Duffus JH, Levy C, Manners DJ (1982) Yeast cell-wall glucans. In: Rose AH, Gareth Morris J (eds) Advances in microbial physiology, vol 23. Academic Press, London, pp 151–181

  12. Eddy AA (1958) The structure of the yeast cell wall. II. Degradation studies with enzymes. Proc Roy Soc 149:425–440

  13. Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

  14. Gorin PAJ, Spencer JFT (1970) Proton magnetic resonance spectroscopy. An aid in identification and chemotaxonomy of yeasts. Adv Appl Microbiol 13:25–89

  15. Horisberger M, Volanthen M (197) Localization of mannan and chitin on thin sections of budding yeasts with gold markers. Arch Microbiol 115:1–7

  16. Huggett ASG, Nixon DA (1957) Enzyme determination of blood glucose. Biochem J 66:12p

  17. Humphries EC (1956) Mineral components and ash analysis. In: Paech K, Tracey MV (eds) Modern methods of plant analysis, vol 1. Springer, Berlin Göttingen Heidelberg, pp 468–502

  18. Johnston IR (1965) The partial acid hydrolysis of a highly destrorotatory fragment of the cell wall of Aspergillus niger. Biochem J 96:659–664

  19. Korn ED, Northcote DH (1960) Physical and chemical properties of polysaccharides and glycoproteins of the yeast cell wall. Biochem J 75:12–17

  20. Kreger DR (1954) Observation on the cell walls of yeasts and some other fungi by X-ray diffraction and solubility tests. Biochim Biophys Acta 13:1–9

  21. Lodder J (1971) The yeasts. North Holland Publishing Company. Amsterdam London

  22. Manners DJ, Meyer MT (1977) The molecular structure of some glucans from the cell walls of Schizosaccharomyces pombe. Carbohydr Res 57:189–203

  23. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380

  24. Partridge SM (1949) Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars. Nature 164:443

  25. Poole RK, Lloyd D (1973) Effect of 2-deoxy-d-glucose on growth and cell walls of Schizosaccharomyces pombe. Arch Mikrobiol 88: 257–272

  26. Roelofsen PA, Hoette I (1951) Chitin in the cell walls of yeasts. Antonie van Leeuwenhoek J Microbiol Serol 17:297–313

  27. Savile DBO (1968) Possible interrelationships between fungal groups. In: Ainsworth GC, Sussman AS (eds) The fungi, vol 3. Academic Press, New York London, pp 649–675

  28. Schneider A (1962) La structure de la membrane chez les Taphrina (Protoascomycètes). C R Acad Sci Paris 255:3453–3455

  29. Schneider A, Dargent R (1972) Ultrastructure de la paroi et des septa du mycélium parasite de Taphrina deformans. C R Acad Sci Paris série D 275:1355–1358

  30. Schneider A, Dargent R (1977) La structure de la paroi ascale chez les Taphrina (Protoascomycètes). Ann Sci Nat Bot 12e série 18: 207–220

  31. Schwimmer S, Bevenue A (1956) Reagent for differentiation of 1,4- and 1,6-linked glucosaccharides. Science 123:543–544

  32. Shields R, Burnett N (1960) Determination of protein bound carbohydrate in serum by a modified anthrone method. Anal Chem 32:885–886

  33. Strauch L (1965) Ultramicrodetermination of nitrogen in biological material. Z Klin Chem 3:165–167

  34. Tracey MV (1955) Chitin. In: Paech K, Tracey MV (eds) Modern methods of plant analysis, vol 2. Springer, Berlin Göttingen Heidelberg, pp 264–274

  35. Trevelyan WE, Proctor DP, Harrison DS (1950) Detection of sugars on paper chromatograms. Nature 166:444–445

  36. Wessels JGH, Sietsma JH (1981) Fungal cell walls: a survey. In: Tanner W, Loewus FA (eds) Encyclopedia of plant physiology, plant carbohydrates II, vol 13 B. Springer, Berlin Heidelberg New York, pp 352–294

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Correspondence to Michel Petit.

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Petit, M., Schneider, A. Chemical analysis of the wall of the yeast form of Taphrina deformans . Arch. Microbiol. 135, 141–146 (1983). https://doi.org/10.1007/BF00408024

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Key words

  • Biochemistry
  • Chimiotaxonomy
  • Fungus wall
  • Taphrina deformans