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Production of emulsifying agent during growth of Pseudomonas cepacia with 2,4,5-trichlorophenoxyacetic acid

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Abstract

The culture supernatant of Pseudomonas cepacia (ATCC 39027) grown on 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was found to contain an agent which can emulsify 2,4,5-T. The emulsion is stable for several hours. The emulsifying agent was produced in response to growth on 2,4,5-T, although some emulsification was observed in culture supernatant of glucose grown cells. The emulsifying agent is most active with 2,4,5-T but has some activity towards other chlorinated compounds such as chlorophenols. In growing culture the emulsifying agent adheres to the cell surface as a slimy layer. The emulsifying agent is believed to have a role in transport of 2,4,5-T into the cells.

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Abbreviations

BSM:

Basal salts medium

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Correspondence to A. M. Chakrabarty.

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Banerjee, S., Duttagupta, S. & Chakrabarty, A.M. Production of emulsifying agent during growth of Pseudomonas cepacia with 2,4,5-trichlorophenoxyacetic acid. Arch. Microbiol. 135, 110–114 (1983). https://doi.org/10.1007/BF00408018

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Key words

  • Emulsifying agent
  • 2,4,5-Trichlorophenoxy-acetic acid
  • Pseudomonas cepacia AC1100