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Archives of Microbiology

, Volume 135, Issue 2, pp 91–97 | Cite as

Characterization of Methanococcus maripaludis sp. nov., a new methanogen isolated from salt marsh sediment

  • W. Jack Jones
  • M. J. B. Paynter
  • R. Gupta
Original Papers

Abstract

A predominant methanogenic bacterium was isolated from salt-marsh sediment near Pawley's Island, South Carolina. A habitat-simulating medium with H2:CO2 as substrate was used for enrichment and isolation. The methanogen is strictly anaerobic, weakly-motile, non-sporeforming, Gram negative, and a pleomorphic coccoid-rod averaging 1.2 by 1.6 μm. Colonies are circular, translucent, pale yellow, and have a smooth surface and an entire edge. The organism is a mesophile, growing between 18 and 47°C, with an optimum near 38°C. The pH optimum for growth is 6.8–7.2, and only formate or a mixture of H2 plus CO2 serve as substrates. Seawater (20–70% v/v) is required, but it can be replaced by 15 mM, or greater, magnesium. Optimal growth occurs with 110 mM sodium. Growth rate is stimulated by selenium (10 μM) but organic compounds (acetate, vitamins, amino acids) are neither stimulatory nor required. The methanogen grows well in autotrophic medium with a doubling time of about 2h. Cells are fragile, are lysed by aqueous solutions of low osmolality and by detergents, and the lack muramic acid. The cell wall is a single electron dense layer. The DNA base composition is 33 mol % guanine plus cytosine. Antigenic relationship of cells and the 16S ribosomal RNA catalog indicate that the salt marsh methanogen is a unique species of Methanococcus, for which we propose the name Methanococcus maripaludis sp. nov.

Key words

Methanococcus maripaludis Marine methanogenic bacteria Methanogen Autotroph Hydrogen oxidation Archaebacteria 

Abbreviations

MPN

Most probable number

SDS

Sodium dodecylsulfate (sodium lauryl sulfate)

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References

  1. Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: Reevaluation of a unique biological group. Microbiol Rev 43:260–296PubMedGoogle Scholar
  2. Barker HA (1940) Studies upon the methane fermentation. IV. The isolation and culture of Methanobacterium omelianskii. Antonie van Leeuwenhock J Microbiol Serol 6:201–220Google Scholar
  3. Bryant MP, Wolin EA, Wolin MJ, Wolfe RS (1967) Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Arch Mikrobiol 59:20–31Google Scholar
  4. Chopra I, Howe TGB (1978) Bacterial resistance to the tetracyclines. Microbiol Rev 42:707–724PubMedGoogle Scholar
  5. Conway de Macario E, Wolin MJ, Macario AJL (1982a) Antibody analysis of relationships among methanogenic bacteria. J Bacteriol 149:316–319PubMedGoogle Scholar
  6. Conway de Macario E, Macario AJL, Wolin MJ (1982b) Specific antisera and immunological procedures for characterization of methanogenic bacteria. J Bacteriol 149:320–328PubMedGoogle Scholar
  7. Fox GE, Magrum LJ, Balch WE, Wolfe RS, Woese CB (1977a) Classification of methanogenic bacteria by 16S ribosomal RNA characterization. Proc Natl Acad Sci USA 74:4537–4541Google Scholar
  8. Fox GE, Pechman KJ, Woese CR (1977b) Comparative cataloging of 16S ribosomal ribonucleic acid: molecular approach to procaryotic systematics. Int J Syst Bacteriol 27:44–57Google Scholar
  9. Hilpert R, Winter J, Hammes W, Kandler O (1981) The sensitivity of archaebacteria to antibiotics. Zbl Bakt Hyg I Abt Orig C2:11–20Google Scholar
  10. Huber H, Thomm M, Konig H, Thies G, Stetter KO (1982) Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen. Arch Microbiol 132:47–50Google Scholar
  11. Hungate RE (1950) The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev 14:1–49PubMedGoogle Scholar
  12. Hungate RE (1969) A roll tube method for cultivation of strict anaerobes. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 3B. Academic Press, New York, pp 117–132Google Scholar
  13. Jones JB, Bowers B, Stadtman TC (1977) Methanococcus vannielii: Ultrastructure and sensitivity to detergents and antibiotics. J Bacteriol 130:1357–1363PubMedGoogle Scholar
  14. Jones JB, Stadtman TC (1977) Methanococcus vannielii: culture and effects of selenium and tungsten on growth. J Bacteriol 130:1404–1406PubMedGoogle Scholar
  15. Jones WJ, Paynter MJB (1980) Populations of methane-producing bacteria and in vitro methanogenesis in salt marsh and estuarine sediments. Appl Environ Microbiol 39:864–871Google Scholar
  16. King JD, White DC (1977) Muramic acid as a measure of microbial biomass in estuarine and marine samples. Appl Environ Microbiol 33:777–783PubMedGoogle Scholar
  17. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218Google Scholar
  18. Paynter MJB, Hungate RE (1968) Characterization of Methanobacterium mobilis sp. nov., isolated from the bovine rumen. J Bacteriol 95:1943–1951PubMedGoogle Scholar
  19. Rommesser JA, Wolfe RS, Mayer F, Spiess E, Walther-Mauruschat A (1979) Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch Microbiol 121:147–153Google Scholar
  20. Sanger F, Brownlee GG, Barrell BB (1965) A two dimensional fractionation procedure for radioactive nucleotides. J Mol Biol 13:373–398PubMedGoogle Scholar
  21. Schildkraut CL, Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol 4:430–443PubMedGoogle Scholar
  22. Stadtman TC, Barker HA (1951) Studies on the methane fermentation. X. A new formate-decomposing bacterium, Methanococcus vannielii. J Bacteriol 62:269–280PubMedGoogle Scholar
  23. Uchida T, Bonen L, Schaup HW, Lewis BJ, Zablen L, Wiese CR (1974) The use of ribonuclease U2 in RNA sequence determination: some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA. J Mol Evol 3:63–77PubMedGoogle Scholar
  24. Whitman WB, Ankwanda E, Wolfe RS (1982) Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol 149:852–863PubMedGoogle Scholar
  25. Woese CR, Sogin M, Stahl D, Lewis BJ, Bonen L (1976) A comparison of the 16 S ribosomal RNAs from mesophilic and thermophilic Bacilli: some modifications in the Sanger method for RNA sequencing. J Mol Evol 7:197–213PubMedGoogle Scholar
  26. Zeikus JG, Bowen VG (1975) Comparative ultrastructure of methanogenic bacteria. Can J Microbiol 21:121–129PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • W. Jack Jones
    • 1
  • M. J. B. Paynter
    • 1
  • R. Gupta
    • 2
  1. 1.Department of MicrobiologyClemson UniversityClemsonUSA
  2. 2.Department of Genetics and DevelopmentUniversity of IllinoisUrbanaUSA

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