An Escherichia coli mutant defective in the NAD-dependent succinate semialdehyde dehydrogenase
Escherichia coli mutants, unable to grown on 4-hydroxyphenylacetate, have been isolated and found to be defective in the NAD-dependent succinate semialdehyde dehydrogenase. When the mutants are grown with 4-aminobutyrate as sole nitrogen source an NAD-dependent succinate semialdehyde dehydrogenase seen in the parental strain is absent but, as in the parental strain, an NADP-dependent enzyme is induced. Growth of the mutants is inhibited by 4-hydroxyphenylacetate due to the accumulation of succinate semialdehyde. The mutants are more sensitive to inhibition by exogenous succinate semialdehyde than is the parental strain. Secondary mutants able to grow in the presence of 4-hydroxyphenylacetate but still unable to use it as sole carbon source were defective in early steps of 4-hydroxyphenylacetate catabolism and so did not form succinate semialdehyde from 4-hydroxyphenylacetate. The gene encoding the NAD-dependent succinate semialdehyde dehydrogenase of Escherichia coli K-12 was located at min 34.1 on the genetic map.
Key wordsEscherichia coli Succinate semialdehyde dehydrogenase Aromatic catabolism Hydroxyphenylacetate Genetic mapping
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- Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12. Edition 6. Microbiol Rev 44:1–56Google Scholar
- Gornall AC, Bardawill CS, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766Google Scholar
- Hareland WA, Crawford RL, Chapman PJ, Dagley S (1975) Metabolic function and properties of 4-hydroxyphenylacetate-1-hydroxylase from Pseudomonas acidovorans. J Bacteriol 121:275–285Google Scholar
- Jakoby WB (1962) Enzymes of 4-aminobutyrate metabolism (bacterial). In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol V. Academic Press, New York, pp 765–778Google Scholar
- Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USAGoogle Scholar