Invertase (β-d-fructofuranosidase, EC 126.96.36.199) activity appeared in cultures and mycelial suspensions of a thermophilic fungus. Thermomyces lanuginosus, only when sucrose or raffinose was added as the carbon source to the medium. The enzyme activity disappeared rapidly following the exhaustion of sugar in the culture medium. Extracellular invertase activity was not detected. The maximal enzyme activity occurred prior to the maximal growth of the fungus. The appearance of invertase activity was dependent on growth. The enzyme activity was not stable in cell-free extracts of mycelia. The lability of the enzyme was also observed in mycelia incubated in the presence of cycloheximide. In the above respects the behaviour of invertase in T. lanuginosus differs from invertase of yeasts and molds (mesophiles) which have been studied.
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Chu FK, Maley F (1980a) Growth phase dependence of invertase mRNA levels in yeast. J Biol Chem 255:6387–6391
Chu FK, Maley F (1980b) The effect of glucose on the synthesis and glycosylation of the polypeptide moiety of yeast external invertase. J Biol Chem 255:6392–6397
Davies R (1953) Enzyme formation in Saccharomyces fragilis. I. Invertase and raffinase. Biochem J 55:484–497
Dodyk F, Rothstein A (1964) Factors influencing the appearance of invertase in Saccharomyces cerevisiae. Arch Biochem Biophys 104:478–486
Elorza MV, Lostau CM, Villanueva JR, Sentandreu R (1977) Invertase messenger ribonucleic acid in Saccharomyces cerevisiae. Biochim Biophys Acta 475:638–651
Esmon B, Novick P, Schekman R (1981) Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast. Cell 25:451–460
Gascon S, Neumann NP, Lampen JO (1968) Comparative study of the properties of the purified internal and external invertase from yeast. J Biol Chem 243:1573–1577
Hill EP, Sussman AS (1964) Development of trehalase and invertase activity in Neurospora. J Bacteriol 88:1556–1566
Iizuka M, Yamamoto T (1972) Studies on invertase of Candida utilis. Part 1: A comparative study of isolation methods of the enzyme. Agric Biol Chem 36:349–355
Lowry OH, Rosebrough NG, Farr AL, Randall RJ (1951) Protein estimation with Folin phenol reagent. J Biol Chem 193:265–275
Mandels GR (1956) Synthesis and secretion of invertase in relation to growth of Myrothecium verrucaria. J Bacteriol 71:684–688
Maruyama Y, Onodera K (1979) Production and some properties of invertase isozymes of Fusarium oxysporum. J Gen Appl Microbiol 25:361–366
Metzenberg RL (1962) A gene affecting the repression of invertase and trehalase in Neurospora. Arch Biochem Biophys 96:468–474
Neuberg C, Roberts IS (1946) Invertase. Scientific Reports Series. No. 4, Sugar Research Foundation, Inc, New York
Novick P, Ferro S, Schekman R (1981) Order of events in the yeast secretary pathway. Cell 25:461–469
Perlman D, Halvorson HO (1981) Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell 25:525–536
Prasad ARS, Maheshwari R (1978a) Growth and trehalase activity in the thermophilic fungus Thermomyces lanuginosus. Proc Indian Acad Sci 87B:231–241
Prasad ARS, Maheshwari R (1978b) Purification and properties of trehalase from the thermophilic fungus Humicola lanuginosa. Biochim Biophys Acta 525:162–170
Prasad ARS, Maheshwari R (1979) Temperature response of trehalase from a mesophilic (Neurospora crassa) and a thermophilic (Thermomyces lanuginosus) fungus. Arch Microbiol 122:275–280
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Trevelyan WE, Procter DP, Harrison JS (1950) Detection of sugars on paper chromatograms. Nature (London) 166:444–445
Trevithick JR, Metzenberg RL (1966) Molecular sieving by Neurospora cell walls during secretion of invertase isoenzymes. J Bacteriol 92:1010–1015
Wickerham LJ (1958) Evidence of the production of extracellular invertase by certain strains of yeasts. Arch Biochem Biophys 76:439–448
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Maheshwari, R., Balasubramanyam, P.V. & Palanivelu, P. Distinctive behaviour of invertase in a thermophilic fungus, Thermomyces lanuginosus . Arch. Microbiol. 134, 255–260 (1983). https://doi.org/10.1007/BF00407798
- Thermomyces lanuginosus
- Thermophilic fungus