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Activity of isocitrate dehydrogenase from three filamentous fungi in relation to osmotic and solute effects

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Abstract

Crude extracts of NADP+-specific isocitrate dehydrogenase (EC 1.1.1.42) were prepared from three filamentous fungi with different tolerances to water stress. There was no difference in the activity of this enzyme extracted from Phytophthora cinnamomi which had been grown on media of osmotic potential of 0 to-2 MPa. Glycerol, proline and glucose caused little or no inhibition of the activity of the enzyme from P. cinnamomi, Penicillium chrysogenum and Chrysosporium fastidium over the range 0 to-10 MPa. Potassium chloride and sucrose were both inhibitory, proportional to the concentration present in the assay buffer and hence inversely proportional to the osmotic potential. Both proline and glycerol were able to relieve the inhibition of the enzyme from P. cinnamomi due to KCl and sucrose, but glycerol did not have this effect on inhibition due to KCl of the other two enzymes. Glycerol was required, for maximal activity of isocitrate dehydrogenase from P. chrysogenum and C. fastidium even in the absence of other solutes. The results are discussed in relation to the growth characteristics of the three species.

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Abbreviations

MPa:

megapascal

1 MPa:

106 Pa; where the pascal is the SI unit of pressure

References

  1. Adler L (1978) Properties of alkaline phosphatase of the halotolerant yeast Debaryomyces hansenii. Biochim Biophys Acta 522:113–121

  2. Borowitzka LJ, Brown AD (1974) The salt relations of marine and halophilic species of the unicellular green alga, Dunaliella. The role of glycerol as a compatible solute. Arch Microbiol 96:37–52

  3. Brown AD (1979) Physiological problems of water stress. In: Shilo M (ed) Strategies of microbial life in extreme environments. Verlag Chemie, Weinheim, pp 65–81

  4. Brown AD, Simpson JR (1972) Water relations of sugar-tolerant, yeasts: the role of intracellular polyols. J Gen Microbiol 72:589–591

  5. Gimmler H, Schirling R (1978) Catron permeability, of the plasmalemma of the halotolerant alga Dunaliella parva. II. Cation content and glycerol concentration of the cells as dependent upon external NaCl concentrations. Z Pflanzenphysiol 87:435–444

  6. Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Ann Rev Plant Physiol 31:149–190

  7. Greenway H, Setter T (1977) Effects of chloride salts at high concentrations on glycolysis in vitro. J Exp Bot 28:545–558

  8. Greenway H, Sims AP (1974) Effects of high concentrations of KCl and NaCl on responses of malate dehydrogenase (decarboxylating) to malate and various inhibitors. Aust J Plant Physiol 1:15–29

  9. Johnson MK, Johnson, EJ, MacElroy RD, Speer HL, Bruff BS (1968) Effects of salts on the halophilic alga Dunaliella viridis. J Bacteriol 95:1461–1468

  10. Lowry OH, Rosebrough MJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275

  11. Luard EJ (1982a) Accumulation of intracellular solutes by two filamentous fungi in response to growth at low steady-state osmotic potential. J Gen Microbiol 128:2563–2574

  12. Luard EJ (1982b) Growth and accumulation of solutes by Phytophthora cinnamomi and other lower fungi in response to changes in external osmotic potential. J Gen Microbiol 128:2583–2590

  13. Luard EJ, Griffin DM (1981) Effect of water potential on fungal growth and turgor. Trans Br Mycol Sco 76:33–40

  14. Miller PM (1955) V-8 juice agar as a general-purpose medium for fungi and bacteria. Phytopath 45:461–462

  15. Norrish RS (1966) An equation, for the activity coefficients and equilibrium relative humidities of water in confectionary syrups. J Food Technol 1:25–29

  16. Pollard A, Wyn Jones RG (1979) Enzyme activities in, concentrated solutions of glycinebetaine and other solutes. Planta 144:291–298

  17. Reeves HC, Rabin R, Wegener, WS, Ajl SJ (1971) Assays of enzymes of the tricarboxylic acid and glyoxalate cycles. In: Norris RJ, Ribbons DW (eds) Methods in microbiology, vol 6A. Academic Press, London, pp 425–462

  18. Robinson RA, Stokes RH (1955) Electrolyte solutions. Academic Press, New York

  19. Scatchard G, Hamer, WJ, Wood SE (1938) Isotonic solutions. I. The chemical potential of water in aqueous solutions of sodium chloride, potassium chloride, sulfuric acid, sucrose, urea and glycerol at 25°. J Amer Chem Soc 60:3061–3070

  20. Smith G (1960) An introduction to industrial mycology. Edward Arnold, London

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Luard, E.J. Activity of isocitrate dehydrogenase from three filamentous fungi in relation to osmotic and solute effects. Arch. Microbiol. 134, 233–237 (1983). https://doi.org/10.1007/BF00407764

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Key words

  • Chrysosporium fastidium
  • Compatible solute
  • Glycerol
  • Isocitrate dehydrogenase
  • Osmotic adjustment
  • Penicillium chrysogenum
  • Phytophthora cinnamomi
  • Proline