Growth-promoting effect of chloro-substituted aliphatic acids in root nodule bacteria
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Summary
Trichloro-, dichloro- and monochloroacetic and α-dichloro-, α-monochloro-and β-monochloropropionic acids as sodium salts in 0.10–1.0mm concentration accelerated the growth of a strain of Rhizobium leguminosarum in a liquid synthetic medium with biotin as sole accessory growth factor. The strongest effect was shown by β-monochloropropionate. Concentrations of 10mm and above were inhibitory. α-Monochlorobutyrate did not accelerate growth.
Ca-pantothenate and its precursor β-alanine strongly improved growth in concentrations of 0.10 to 1.0 p.p.m. (0.001–0.011mm β-alanine) and masked the effect of the chloro-substituted organic acids which seemed to function as more or less inferior substitutes for β-alanine.
The effect of β-alanine was approximately ten times greater than that of β-monochloropropionate which possibly owed its relatively strong activity to its close structure analogy with β-alanine.
Two other strains of rhizobia showed a moderate response to trichloroacetate and dichloropropionate, while others failed to do so and grew abundantly with biotin alone.
Keywords
Biotin Rhizobium Sodium Salt Synthetic Medium DichloroPreview
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References
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