The cyanobacterium Oscillatoria agardhii Gomont was cultivated with a diurnal light-dark cycle (photoperiod 16 h) in continuous culture. There were found to be large differences in specific synthesis rates of the different biopolymers. The specific rates of change of proteins and nucleic acids (except DNA) matched the dilution rate, both in the light and in the dark period. Carbohydrates were synthesized and stored at a very high rate during the photoperiod, and were metabolized for the provision of energy, and for biosynthesis of other biopolymers in the dark. Cell counts showed no evidence of phased synchrony, although this conclusion was contradicted by changes in DNA and pigments.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Clark, D. J.: The regulation of DNA replication and cell division in Escherichia coli B/r. Cold Spring Harbor Symp. Quant. Biol. 33, 823–838 (1968)
Donachie, W. D., Martin, D. T. M., Begg, K.J.: Independence of cell division and DNA replication in Bacillus subtilis. Nature (New Biology) 231, 274–276 (1971)
Dring, M. J.: Reproduction. In: Algal physiology and biochemistry, Bot. Monogr., Vol. 10 (W. D. P. Stewart, ed.), pp. 814–837. Oxford, London, New York: Blackwell Sc. Publ. 1974
Fogg, G. E., Stewart, W. D. P., Fay, P., Walsby, A. E.: The blue-green algae. London, New York: Academic Press 1973
Foy, R. H., Gibson, C. E., Smith, R. V.: The influence of daylength, light intensity and temperature on the growth of planktonic blue-green algae. Br. Phycol. J. 11, 151–163 (1976)
Fuhs, G. W.: Cytochemical examination of blue-green algae. In: The biology of the blue-green algae. Bot. Monogr., Vol. 9 (N. G. Carr, B. A. Whitton, eds.), pp. 117–143. Oxford, London, New York: Blackwell Sc. Publ. 1973
Gons, H. J., Barug, D., Mur, L. R.: Effects of a light-dark cycle on the growth of Scenedesmus protuberans Fritsch in light-limited continuous cultures. Hydrobiol. Bull. 9, 71–80 (1975)
Helmstetter, C. E., Pierucci, O.: Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J. Bacteriol. 95, 1627–1633 (1968)
Herdman, M., Faulkner, B. M., Carr, N. G.: Synchronous growth and genome replication in the blue-green alga Anacystis nidulans. Arch. Microbiol. 73, 238–249 (1970)
Loogman, J. G., Van Liere, L.: An improved method for measuring irradiance in algal cultures. Mitt. Int. Ver. Theor. Angew. Limnol. 20, 2322–2328 (1978)
Lorenzen, H., Hesse, M.: Synchronous cultures. In: Algal physiology and biochemistry, Bot. Monogr., Vol. 10 (W. D. P. Stewart, ed.), pp. 894–908. Oxford, London, New York: Blackwell Sc. Publ. 1974
Mann, N., Carr, N. G.: Control of macromolecular composition and cell division in the blue-green alga Anacystis nidulans. J. Gen. Microbiol. 83, 399–405 (1974)
Mann, N., Carr, N. G.: Coupling between the initiation of DNA replication and cell division in the blue-green alga Anacystis nidulans. Arch. Microbiol. 112, 95–98 (1977)
Rhee, G-Y., Gotham, I. J.: Use of the cyclostat for phytoplankton ecology. In: Continuous culture of cells (P. Calcott, ed.). Cleveland, Ohio: CRC Press (1981)
Van Gemerden, H.: Coexistence of organisms competing for the same substrate: An example among the purple sulphur bacteria. Microb. Ecol. 1, 104–119 (1974)
Van Liere, L., Loogman, J. G., Mur, L. R.: Measuring light-irradiance in cultures of phototrophic micro-organisms. FEMS Microbiol. Lett. 3, 161–164 (1978)
Van Liere, L., Mur, L. R.: Light-limited cultures of the blue-green alga Oscillatoria agardhii. Mitt. Int. Ver. Theor. Angew. Limnol. 21, 158–167 (1978)
Van Liere, L.: On Oscillatoria agardhii Gomont, experimental ecology and physiology of a nuisance bloom-forming cyanobacterium, Thesis, Universiteit van Amsterdam. Zeist. De Nieuwe Schouw Press 1979
Walsby, A. E.: Structure and function of gas vacuoles. Bacteriol. Rev. 36, 1–32 (1972)
About this article
Cite this article
van Liere, L., Mur, L.R., Gibson, C.E. et al. Growth and physiology of Oscillatoria agardhii gomont cultivated in continuous culture with a light-dark cycle. Arch. Microbiol. 123, 315–318 (1979). https://doi.org/10.1007/BF00406668
- Oscillatoria agardhii
- Biopolymer synthesis
- Continuous culture
- Diurnal light-dark cycle
- Light-energy limitation
- Phased synchrony