Archives of Microbiology

, Volume 128, Issue 2, pp 215–221

An electron microscopic study on the mating tube formation in the heterobasidiomycete Tremella mesenterica

  • Aiko Hirata
  • Eiko Tsuchiya
  • Sakuzo Fukui
  • Kenji Tanaka
Article

Abstract

Ultrastructure of the mating tube formed in yeast haplont of the heterobasidiomycete Tremella mesenterica was studied by electron microscopy. Cell wall of the mating tube emerged as evagination of the inner layers, rupturing outer layers of the mother cell wall. Comparison with budding cells suggested that the tube emergence place at bud scar and the process of tube emergence was the same as that of bud emergence. Electron transparent vesicles of 0.1 μm diameter were scattered in the cytoplasm of the mating tube. Nucleus-associated organelle was located at one side of the nuclear envelope which extended towards the mating tube. A few microtubules were detected in the mating tube, but their association with a nucleus was not clear. The cytoplasmic structure of the mating tube was discussed in comparison with that of hyphae of the filamentous fungi.

Key words

Mating tube Microtubule Tremella Ultrastructure Yeast 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe, K., Kusaka, I., Fukui, S.: Morphological change in the early stages of the mating process of Rhodosporidium toruloides. J. Bacteriol. 122, 710–718 (1975)PubMedGoogle Scholar
  2. Abe, K., Tsuchiya, E., Kusaka, I., Fukui, S., Kusanagi, A.: Nuclear behavior of Rhodosporidium toruloides, a heterobasidiomycetous yeast. J. Gen. Appl. Microbiol. 23, 175–181 (1977)Google Scholar
  3. Abe, K.: Studies on sexual differentiation of the heterobasidiomycetous yeast, Rhodosporidium toruloides. Doctor thesis, Tokyo University, Tokyo 1979Google Scholar
  4. Bandoni, R. J., Bisalputra, A. A.: Budding and fine structure of Tremella mesenterica haplonts. Can. J. Bot. 49, 27–30 (1971)Google Scholar
  5. Brown, J. P., Edwards, M. R.: Observations on budding cells of Torulopsis glabrata. Can. J. Microbiol. 17, 609–616 (1971)PubMedGoogle Scholar
  6. Byers, B., Goetsch, L.: Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. J. Bacteriol. 124, 511–523 (1975)PubMedGoogle Scholar
  7. Dustin, P.: In: Microbules, pp. 226–256. Berlin, Heidelberg, New York: Springer 1978Google Scholar
  8. Fernández, J. A., Hess, W. M.: Sporidial reproduction in Sorosporium consanguineum: Wall ontogeny Mycologia 70, 814–820 (1978)Google Scholar
  9. Girbardt, M.: Ultrastructure and dynamics of the moving nucleus. In: Aspects of cell motility. 22nd Symp. Soc. Exp. Biol. (P. L. Miller, ed.), pp. 249–259. Cambridge: Cambridge University Press 1968Google Scholar
  10. Girbardt, M., Hädrich, H.: Ultrastruktur des Pilzkernes. III. Genese des Kern-assoziierten Organelles (NAO=KCE). Z. Allg. Mikrobiol. 15, 157–173 (1975)PubMedGoogle Scholar
  11. Grove, S. N.: The cytology of hyphal tip growth. In: The filamentous fungi. Vol. 3 (J. E. Smith, D. R. Berry, eds.), pp. 28–50. London: Edw. Arnold 1978Google Scholar
  12. Heath, I. B., Heath, M. C.: Microtubules and organelle movements in the rust fungus Uromyces phaseoli var. vignae. Cytobiologie 16, 393–411 (1978)PubMedGoogle Scholar
  13. Kreger-van Rij, N. J. W., Veenhuis, M.: A comparative study of the cell wall structure of basidiomycetous and related yeasts. J. Gen. Microbiol. 68, 87–95 (1971a)Google Scholar
  14. Kreger-van Rij, N. J. W., Veenhuis, M.: Bipolar budding in yeasts —an electron microscope study. Antonie van Leeuwenhock 37, 125–136 (1971b)Google Scholar
  15. Lara, S. L., Bartnicki-Garcia, S.: Cytology of budding in Mucorrouxii: Wall ontogeny. Arch. Microbiol. 97, 1–16 (1974)Google Scholar
  16. Marchant, R., Smith, D. G.: Bud formation in Saccharomyces cerevisiae and a comparison with the mechanism of cell division in other yeasts. J. Gen. Microbiol. 53, 163–169 (1968)PubMedGoogle Scholar
  17. Raudaskoski, M.: Occurrence of microtubules in the hyphae of Schizophyllum commune during intercellular nuclear migration. Arch. Mikrobiol. 86, 91–100 (1972)PubMedGoogle Scholar
  18. Shannon, J. L., Rothman, A. H.: Transverse septum formation in budding cells of the yeastlike fungus Candida albicans. J. Bacteriol. 106, 1026–1028 (1971)PubMedGoogle Scholar
  19. Talens, L. T., Miranda, M., Miller, M. W.: Electron micrography of bud formation in Metschnikowia krissii. J. Bacteriol. 114, 413–423 (1973)Google Scholar
  20. Trinci, A. P. J.: The duplication cycle and vegetative development in moulds. In: The filamentous fungi, Vol. 3 (J. E. Smith, D. R. Berry, eds.), pp. 132–163. London: Edw. Arnold 1978Google Scholar
  21. Tsuchiya, E., Fukui, S.: Biological activities of sex hormone produced by Tremella mesenterica. Agric. Biol. Chem. 42, 1089–1091 (1978)Google Scholar
  22. Tsuchiya, E.: Biochemical studies on sexual differentiation of the heterobasidiomycetous yeast, Tremella mesenterica. Doctor thesis, Tokyo University, Tokyo 1979Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Aiko Hirata
    • 1
  • Eiko Tsuchiya
    • 1
  • Sakuzo Fukui
    • 1
  • Kenji Tanaka
    • 2
  1. 1.Institute of Applied MicrobiologyUniversity of TokyoTokyoJapan
  2. 2.Institute of Medical MycologyNagoya University School of MedicineNagoyaJapan

Personalised recommendations