Archives of Microbiology

, Volume 118, Issue 1, pp 55–60 | Cite as

Regulation of proteolytic enzymes in axenically grown myxamoebae of Physarum polycephalum

  • Annegret Haars
  • Clare H. R. McCullough
  • Aloys Hüttermann
  • Jennifer Dee


The cultivation of Physarum polycephalum amoebae in two media with different protein contents revealed a regulation of aminopeptidases and proteases depending on the albumin content of the medium: in growing amoebae and plasmodia the aminopeptidases have similar isoenzyme patterns and relative activities against nitroanilides. One alanine and four leucine aminopeptidase isoenzymes were found within the slightly acid pH range. During growth amoebae secrete—different from plasmodia—leucine aminopeptidase into the medium with low protein content. In an albumin-rich medium additional alanine aminopeptidase activity was found. Out of nine plasmodial proteases four were found in amoebae too. Only one band (pI 3.6) was present in the protein-poor medium. No protease activity could be detected in the proteinrich medium.

Key words

Physarum polycephalum Amoebae Aminopeptidases Acid proteases Regulation Development Differential gene activity 



bovine serum albumin


semi-defined (medium with low protein content; Table 1)


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Annegret Haars
    • 1
  • Clare H. R. McCullough
    • 2
  • Aloys Hüttermann
    • 1
  • Jennifer Dee
    • 2
  1. 1.Forstbotanisches Institut der Universität GöttingenGöttingenFederal Republic of Germany
  2. 2.Department of GeneticsThe UniversityLeicesterEngland

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