Comparison of the urinary excretion of arsenic metabolites after a single oral dose of sodium arsenite, monomethylarsonate, or dimethylarsinate in man

  • J. P. Buchet
  • R. Lauwerys
  • H. Roels
Original Papers

Summary

The urinary elimination of the metabolites of arsenic has been followed up as a function of time in volunteers who ingested a single oral dose of arsenic (500 μg As) either as sodium arsenite (Asi), monomethylarsonate (MMA), or cacodylate (DMA). The excretion rate increased in the order Asi < DMA < MMA. After 4 days, the amount of arsenic excreted in urine represents 46, 78, and 75% of the ingested dose in the case of Asi, MMA and DMA, respectively. With regard to the in vivo biotransformations, it is concluded that DMA is excreted unchanged; MMA is slightly (13%) methylated into DMA while roughly 75% of the arsenic excreted after ingestion of Asi is methylated arsenic (about 1/3 as MMA and about 2/3 as DMA).

Key words

Arsenic Sodium arsenite Monomethylarsonate Dimethylarsinate Human metabolism 

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References

  1. Andreae MO (1977) Determination of arsenic species in natural waters. Anal Chem 49:820–823Google Scholar
  2. Braman RS, Foreback CC (1973) Methylated forms of arsenic in the environment. Science 182:1247–1249Google Scholar
  3. Buchet JP, Lauwerys R, Roels H (1980) Comparison of several methods for the determination of arsenic compounds in water and in urine. Int Arch Occup Environ Health 46:11–29Google Scholar
  4. Committee on Medical and Biological Effects of Environmental Pollutants, Arsenic. National Academy of Sciences, Washington, DC, 1977Google Scholar
  5. Crecelius EA (1974) The geochemistry of arsenic and antimony in Puget Sound and Lake Washington, Washington. PhD Thesis, University of Washington, Seattle, WashingtonGoogle Scholar
  6. Crecelius EA (1977) Changes in the chemical speciation of arsenic following ingestion by man. Environ Health Perspect 19:147–150Google Scholar
  7. Edmonds JS, Francesconi KA (1976) Estimation of methylated arsenicals by vapor generation atomic absorption spectrometry. Anal Chem 48:2019Google Scholar
  8. Fairchild EJ, Lewis RJ, Tatken RL (1977) Registry of toxic effects of chemical substances, US Department of Health, Education, and Welfare. National Institute for Occupational Safety and Health. Cincinnati, OHGoogle Scholar
  9. Feldman C (1979) Improvements in the arsine accumulation-helium glow detector procedure for determining traces of arsenic. Anal Chem 51:664–669Google Scholar
  10. Henry FT, Thorpe TM (1980) Determination of arsenic (III), arsenic (V), monomethylarsonate, and dimethylarsinate by differential pulse polarography after separation by ion exchange chromatography. Anal Chem 52:80–83Google Scholar
  11. Lakso JU, Rose LJ, Peoples SA, Shirachi DY (1979) A colorimetric method for the determination of arsenite, arsenate, mono methylarsonic acid, and dimethylarsinic acid in biological and environmental samples. J Agric Food Chem 27:1229–1233Google Scholar
  12. Lauwerys RR, Buchet JP, Roels H (1979) The determination of trace levels of arsenic in human biological materials. Arch Toxicol 41:239–247Google Scholar
  13. Mappes R (1977) Versuche zur Ausscheidung von Arsen im Urin. Int Arch Occup Environ Health 40:267–272Google Scholar
  14. Nordberg GF, Pershagen G, Lauwerys R (1979) Inorganic arsenic. Toxicological and epidemiological aspects. Report to the Commission of European Communities. Department of Community Health and Environmental Medicine. Odense University, OdenseGoogle Scholar
  15. Odanaka Y, Matano O, Goto S (1978) Identification of dimethylated arsenic by gas chromatography mass spectrometry in blood, urine, and feces of rats treated with ferric methanearsonate. J Agric Food Chem 26:505–507Google Scholar
  16. Pershagen G, Vahter M (1979) Arsenic. A toxicological and epidemiological appraisal. Rapport SNVPM 1128 Naturvårdsverket. Departments of Environmental Hygiene of the Karolinska Institute and the National Swedish Environment Protection Board, StockholmGoogle Scholar
  17. Pinto SS, Varner MO, Nelson KW, Labbe AL, Lowell D (1976) Arsenic trioxide absorption and excretion in industry. J Occup Med 18:667–680Google Scholar
  18. Pomroy C, Charbonneau SM, McCullough RS, Tam GKH (1980) Human retention studies with 14As. Toxicol Appl Pharmacol 53:550–556Google Scholar
  19. Smith TJ, Crecelius EA, Reading JC (1977) Airbone arsenic exposure and excretion of methylated arsenic compounds. Environ Health Perspect 19:89–93Google Scholar
  20. Stevens JT, Di Pasquale LC, Farmer JD (1979) The acute inhalation toxicology in the technical grade organo arsenial herbicides, cacodylic acid, and disodium methane arsonic acid. A route comparison. Bull Environ Contam Toxicol 21:304–311Google Scholar
  21. Talmi Y, Bostick DT (1975) The determination of arsenic and arsenicals. J Chromatogr Sci 13:231–237Google Scholar
  22. Tam KH, Charbonneau SM, Bryce F, Lacroix G (1978) Separation of arsenic metabolites in dog plasma and urine following intravenous injection 74As. Anal Biochem 86:505–511Google Scholar
  23. Tam KH, Charbonneau SM, Bryce F, Pomroy C, Sandi E (1979) Metabolism of inorganic arsenic (74As) in humans following oral ingestion. Toxicol Appl Pharmacol 50:319–322Google Scholar
  24. Wagner SL, Weswig P, Corvallis O (1974) Arsenic in blood and urine of forest workers as indices of exposure to cacodylic acid. Arch Environ Health 28:77–79Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • J. P. Buchet
    • 1
  • R. Lauwerys
    • 1
  • H. Roels
    • 1
  1. 1.Unité de Toxicologie Industrielle et MédicaleUniversité de LouvainBrusselsBelgium

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