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Liver changes under combined effect of working environmental factors

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Liver changes after separate and combined action of vibration (whole body, 100 Hz, 0.1 mm amplitude) and other factors: noise (white noise 105 dB/A), heat (35°C, humidity 45–55% and air velocity 0.2–0.3 ms) and lead (lead acetate, 20 mg/kg) were studied in white rats. The exposure lasted for 2 h daily during 10 days (lead was daily applied per os in a water solution). After the treatment in liver homogenates the activity of SucDH, LDH, and ATP-ase, as well as the quantity of soluble proteins (SP) and -SH gr were determined. In fresh frozen liver slices the activity of SucDH, LDH, and ATP-ase were investigated. Liver samples were studied by light and electromicroscopy. The results show that vibration alone did not produce marked changes, but when the other factors acted simultaneously, more expressed alterations in the liver were found. The most pronounced changes were obtained after vibration and lead effect. The histological, histochemical, and electron-microscopic findings support the biochemical data about certain disturbances in the energy supply and utilization in the liver tissue.

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  1. 1

    Antov G, Ivanovich E, Zaprianov Z (1978) Combined effect of vibration and lead on rat liver and kidney metabolism (in Russian). Gig Tr Prof Zabol 5:54–56

  2. 2

    Gohlke R (1975) Kombinierte Umweltfaktoren am Arbeitsplatz im morphologischen Bild. Z Aerztl Fortbild (Jena) 69:505–515

  3. 3

    Ericson J (1972) Mechanism of cellular autophagy. In: Dingle JT, Fell HB (eds) Lysosomes in biology and pathology. North Holland, Amsterdam, pp 145–391

  4. 4

    Ivanovich E, Antov G (1977) Biochemical changes in some parenchymal organs, due to combined vibration and noise action. Proceedings of the International Congress on Acoustics, Madrid, 4.–9. July

  5. 5

    Kirk J, Laursen T (1955) Dehydrogenase activities of human aortic tissues determined with triphenyl tetrasolium technique. J Gerontol 10:18–25

  6. 6

    Kustov VV, Tiunov LA, Vasilev GA (1975) Combined action of industrial agents (in Russian). Moscow Med

  7. 7

    Lind H (1964) About the acitivy of urease and ATP-ase in subcellular fractions of ventricular mucosis (in Russian). Biochimia (Moscow) 29:191–195

  8. 8

    Lowry Q et al (1946) Quantity estimation of proteins in biological materials. J Biol Chem 162:421–428

  9. 9

    Michailov MM (1972) Combined effect of chemical and physical environmental factors (in Russian). Sverdlovsk, pp 3-10

  10. 10

    Nachlas M, Tsou L, Souza E et al (1957) Cytochemical demonstration of SucDH by use of a new p-nitrophenyl substituted ditetrasole. J Histochem Cytochem 5:420–436

  11. 11

    Padycula A, Herman E (1955) Factors affecting the activity of ATP-ase and other phosphatases as measured by histochemical techniques. J Histochem Cytochem 3:161–169

  12. 12

    Staron T, et al (1971) Determination of sulfhydryl groups in proteins. CR Acad Sci [D] (Paris) 253:3109–3111

  13. 13

    WHO working document on internationally recommended health based permissible levels for occupational exposure to Cd, Ph, Mn, Hg. WHO, Geneva, 1979

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Ivanovich, E., Antov, G. & Kazakova, B. Liver changes under combined effect of working environmental factors. Int. Arch Occup Environ Heath 48, 41–47 (1981). https://doi.org/10.1007/BF00405930

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Key words

  • Liver
  • Vibration
  • Combined exposure
  • Biochemical investigation
  • Morphological study