Advertisement

Current Genetics

, Volume 14, Issue 1, pp 43–49 | Cite as

Physical mapping of the mitochondrial genome of the cultivated mushroom Agaricus brunnescens (= A. bisporus)

  • William E. A. Hintz
  • James B. Anderson
  • Paul A. Horgen
Original Articles

Summary

Mitochondrial (mt) DNA from the commercial mushroom Agaricus brunnescens Peck [= A. bisporus (Lange) Imbach] was purified by cesium chloride/bisbenzimide gradient centrifugation. A physical map of the mtDNA fragments produced by BamHI, EcoRl, and PvuII digestion was generated by filter hybridizations with radiolabelled BamHI mtDNA probes. The A. brunnescens mtDNA was a circular molecule 136 kilo-basepairs (kbp) in length and contained an inverted repeat between 4.6 and 9.2 kbp in size. Orientational isomers of the mitochondrial genome were not detected. The positions of six genes were located on the A. brunnescens mtDNA map by heterologous hybridization. No coding function has yet been ascribed to the inverted repeat. The large rRNA gene was located on the smaller single copy region. The genes for cytochrome b, cytochrome oxidase (subunit III), ATPase (subunits 8 and 6) and the small rRNA were located on different regions of the larger single copy region.

Key words

Agaricus MtDNA Restriction map Inverted repeat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adelberg EA, Bergquist P (1972) Proc Natl Acad Sci USA 69:2061–2065Google Scholar
  2. Boyd D, Hobman TC, Gruenke SA, Klassen GR (1984) Can J Biochem Cell Biol 62:571–576Google Scholar
  3. Castle AJ, Horgen PA, Anderson JB (1987) Appl Environ Microbiol 53:816–822Google Scholar
  4. Clark-Walker GD, McArthur CR, Sriprakash KS (1981) J Mol Biol 247:399–415Google Scholar
  5. Collins RA, Lambowitz AM (1983) Plasmid 9:53–70Google Scholar
  6. Connerton IF, Ray MK, Lancashire WE, Griffiths DE (1984) Mol Gen Genet 193:149–152Google Scholar
  7. Dawson AJ, Hodge TP, Isaac PG, Leaver CJ, Lonsdale DM (1986) Curr Genet 10:561–564Google Scholar
  8. Dujon B (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983: nucleo-mitochondrial interactions. de Gruyter, Berlin New York, pp 4–11Google Scholar
  9. Heckman JE, Raj Bhandary UL (1979) Cell 17:583–595Google Scholar
  10. Hintz WEA, Mohan M, Anderson JB, Horgen PA (1985) Curr Genet 9:127–132Google Scholar
  11. Hudspeth MES, Shumard DS, Tatti KM, Grossman LI (1980) Biochem Biophys Acta 610:221–228Google Scholar
  12. Hudspeth MES, Shumard DS, Bradford CJR, Grossman LI (1983) Proc Natl Acad Sci USA 80:142–146Google Scholar
  13. Malloch D, Castle A, Hintz WEA (1987) Mycologia 79:839–846Google Scholar
  14. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  15. McNabb S, Boyd D, Belkhiri A, Dick MW, Klassen GR (1987) Curr Genet 12:205–208Google Scholar
  16. Mery-Drugeon E, Crouse EJ, Schmitt JM, Bohnert H, Bernard G (1981) Eur J Biochem 114:577–583Google Scholar
  17. Palmer JD (1985) In: MacIntyre RJ (ed) Monographs in evolutionary biology: molecular evolutionary genetics. Plenum Publishing, New York, NY, pp 13–14Google Scholar
  18. Palmer JD, Stein DB (1982) Curr Genet 5:165–170Google Scholar
  19. Palmer JD, Thomson WF (1982) Cell 29:537–550Google Scholar
  20. Palmer JD, Singh GP, Pillay DTN (1983) Mol Gen Genet 190:13–19Google Scholar
  21. Royse DJ, May B (1982) Mycologia 74:93–102Google Scholar
  22. Shumard DS, Grossman LI, Hudspeth MES (1986) Mol Gen Genet 202:16–23Google Scholar
  23. Specht CA, Novotny CP, Ullrich RC (1983) Exp Mycol 7:336–343Google Scholar
  24. Stevens RB (1981) Mycology Guidebook. University of Washington Press, Seattle, p 712Google Scholar
  25. Taylor JW, Smolich BD (1985) Curr Genet 9:597–603Google Scholar
  26. Vieira J, Messing J (1982) Gene 19:259–268Google Scholar
  27. Wallace DC (1982) Microbiol Rev 46:208–240Google Scholar
  28. Ward BL, Anderson RS, Bendich AJ (1981) Cell 35:793–803Google Scholar
  29. Weber CA, Hudspeth MES, Moore GP, Grossman LI (1986) Curr Genet 10:515–525Google Scholar
  30. Wills JW, Troutman WB, Riggsby WS (1985) J Bacteriol 164:7–13Google Scholar
  31. Zolan M, Pukkila P (1986) Mol Cell Biol 6:195–200Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • William E. A. Hintz
    • 1
  • James B. Anderson
    • 1
  • Paul A. Horgen
    • 1
  1. 1.Mushroom Research Group, Center for Plant Biotechnology, Department of BotanyUniversity of TorontoMississaugaCanada

Personalised recommendations