Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Light and electron microscopy of Sondermann's channels in the human trabecular meshwork

  • 20 Accesses

  • 15 Citations

Summary

Channels identified as Sondermann's “inner canaliculi” were found in the trabecular meshwork of both infant and adult eyes of the human by means of light and electron microscopy. They were lined by endothelia, and were located in the endothelial meshwork. They clearly opened into Schlemm's canal. The structure of these channels has been described. Conclusive information, with respect to the relation of these channels to the intertrabecular spaces and, therefore, the anterior chamber, has not been obtained. However, study of serial electron micrographs strongly suggests that the channels communicated with such spaces.

Light microscopy of this area showed that the channels had a complicated course, and that some of them were more slit-like in cross section than tubules.

Zusammenfassung

Im Trabekelwerk menschlicher Augen wurden licht- und elektronenmikroskopisch Kanälchen nachgewiesen, die als Sondermannsche Innenkanälchen identifiziert werden konnten. Sie sind mit Endothel ausgekleidet, befinden sich im Trabeculum cribriforme und weisen eine Öffnung zum Schlemmschen Kanal auf. Die Feinstruktur dieser Kanälchen wird beschrieben. Bisher konnte jedoch nicht endgültig abgeklärt werden, ob diese Kanälchen mit den intertrabekulären Zwischenräumen in Beziehung stehen und damit auch mit der Vorderkammer verbunden sind. Elektronenmikroskopische Serienuntersuchungen lassen jedoch vermuten, daß diese Kanälchen mit den genannten Zwischenräumen in direkter Verbindung stehen.

Lichtmikroskopische Untersuchungen dieses Gebietes zeigen, daß diese Kanälchen einen komplizierten Verlauf nehmen und daß einige von ihnen im Querschnitt eher schlitzförmig als röhrenförmig aussehen.

This is a preview of subscription content, log in to check access.

References

  1. Asayama, J.: Zur Anatomie des Ligamentum pectinatum. Albrecht v. Graefes Arch. Ophthal. 53, 113 (1901).

  2. Ashton, N., A. Brini, and R. Smith: Anatomical studies of the trabecular meshwork of the normal human eye. Brit. J. Ophthal. 40, 257 (1956).

  3. Bárány, E. H., and J. W. Rohen: Glaucoma in monkeys (Ceropithecus aethiops). Arch. Ophthal. 69, 630 (1963).

  4. Cahn, P. H., and W. H. Havener: Factors of importance in traumatic hyphema with particular reference to and study of routes of absorption. Amer. J. Ophthal. 55, 591 (1963).

  5. Carpenter, R. L.: In: Glaucoma, Transactions of the Third Conference (ed. F. W. Newell), p. 67. New York: Josiah Macy, Jr. Foundation 1958.

  6. Caulfield, J. B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. biophys. biochem. Cytol. 3, 827 (1957).

  7. Erlandson, R. A.: A new Maraglas, D. E. R. ® 732, embedment for electron microscopy. J. Cell Biol. 22, 704 (1964).

  8. Fine, B. S.: Observations on the drainage angle in man and rhesus monkey: A concept of the pathogenesis of chronic simple glaucoma. Invest. Ophthal. 3, 609 (1964).

  9. Flocks, M.: The anatomy of the trabecular meshwork as seen in tangential section. Arch. Ophthal. 56, 708 (1956).

  10. François, J., A. Neetens, and J. M. Collette: Microradiographic study of the inner wall of Schlemm's canal. Amer. J. Ophthal. 40, 491 (1955).

  11. Friedenwald, J. S.: Circulation of the aqueous. V. Mechanism of Schlemm's canal. Arch. Ophthal. 16, 65 (1936).

  12. Garron, L. K., and M. L. Feeney: Electron microscopic studies of the human eye. II. Study of the trabeculae by light and electron microscopy. Arch. Ophthal. 62, 966 (1959).

  13. Holmberg, A.: The fine structure of the inner wall of Schlemm's canal. Arch. Ophthal. 62, 956 (1959).

  14. Holmberg, Å. S.: Schlemm's canal and the trabecular meshwork. An electron microscopic study of the normal structure in man and monkey (Cerocopithecus aethiops). Docum. ophthal. (Den Haag) 19, 339 (1965).

  15. Hørven, I.: A radioautographic study of erythrocyte resorption from the anterior chamber of the human eye. Acta ophthal. (Kbh.) 42, 600 (1964).

  16. Huggert, A.: Pore size in the filtration angle of the eye. Acta ophthal. (Kbh.) 33, 271 (1955).

  17. Iwamoto, T.: Light and electron microscopy of the presumed elastic components of the trabeculae and scleral spur of the human eye. Invest. Ophthal. 3, 144 (1964).

  18. —: In: An atlas of anterior chamber angle (by S. Shikano and K. Shimizu), p. 11, Fig. 14. Tokyo: Nanzando 1965.

  19. —, and G. K. Smelser: Electron microscope studies on the mast cells and blood and lymphatic capillaries of the human corneal limbus. Invest. Ophthal. 4, 815 (1965).

  20. Karg, S. J., L. K. Garron, M. L. Feeney, and W. K. McEwen: Perfusion of human eyes with latex microspheres. Arch. Ophthal. 61, 68 (1959).

  21. Loewenstein, A.: The anterior draining system in the human eye. Ophthalmologica (Basel) 122, 257 (1951).

  22. Luft, J. H.: Improvements in epoxy resin embedding methods. J.biophys. biochem. Cytol. 9, 409 (1961).

  23. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208 (1963).

  24. Rohen, J., u. H.-H. Unger: Feinbau und Reaktions-Möglichkeiten des Trabekelwerkes im menschlichen Auge. Anat. Anz. 104, 287 (1957).

  25. Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19 (1963).

  26. Shikano, S., and T. Iwamoto: Electron- and light-microscopic studies on the human trabecular meshwork. Jap. Mschr. prakt. Augenheilk. 56, 765 (1962).

  27. Sondermann, R.: Beitrag zur Entwicklung und Morphologie des Schlemmschen Kanals. Albrecht. v. Graefes Arch. Ophthal. 124, 521 (1930).

  28. —: Über Entstehung, Morphologie und Funktion des Schlemmschen Kanals. Acta ophthal. (Kbh.) 11, 280 (1933).

  29. Speakman, J. S.: Drainage channels in the trabecular wall of Schlemm's canal. Brit. J. Ophthal. 44, 513 (1960).

  30. Spelsberg, W. W., and G. B. Chapman: Personal communication.

  31. Swindle, P. F.: The principal drainage channels of the eye. Arch. Ophthalm. 17, 420 (1937).

  32. Theobald, G. D.: Schlemm's canal: Its anastomoses and anatomic relations. Trans. Amer. ophthal. Soc. 32, 574 (1934).

  33. —: Further studies on the canal of Schlemm: Its anastomoses and anatomic relations. Amer. J. Ophthal. 39, 65 (Pt. II) (1955).

  34. —: The limbal area; with particular reference to the trabecular meshwork in health and disease. Amer. J. Ophthal. 50, 543 (1960).

  35. Unger, H.-H.: Zur Gestalt des Filterwerkes der Kammerbucht. Albrecht v. Graefes Arch. Ophthal. 158, 509 (1957).

  36. —, and J. Rohen: Studies on the histology of the inner wall of Schlemm's canal. Amer. J. Ophthal. 48, 204 (1959).

  37. Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475 (1958).

  38. Yamashita, T., and D. A. Rosen: The elastic tissue of primate trabecular meshwork. A histologic and electron microscopic study. Invest. Ophthal. 3, 85 (1964).

Download references

Author information

Additional information

This investigation was conducted under a Fight for Sight Postdoctoral Research Fellowship of the National Council to Combat Blindness, Inc., New York, N.Y.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Iwamoto, T. Light and electron microscopy of Sondermann's channels in the human trabecular meshwork. Albrecht von Graefes Arch. Klin. Ophthalmol. 172, 197–212 (1967). https://doi.org/10.1007/BF00405390

Download citation

Keywords

  • Public Health
  • Microscopy
  • Electron Microscopy
  • Light Microscopy
  • Electron Micrographs