High performance liquid chromatographic procedure for quantitative determination of urinary delta-aminolevulinic acid as indices of lead exposure
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A high performance liquid chromatographic method for the determination of delta-aminolevulinic acids (ALA) in urine, is described. 2-Methyl-3 carbmethoxy-4-(3-propionic acid) pyrrole was produced by the condensation of ALA with methylacetoacetate (MAA) while heating. The methylacetoacetate could be replaced by ethylacetoacetate. The ALA pyrrole thus obtained was injected into high performance liquid chromatography (HPLC). A stainless-steel column packed with octadecyl silanized silica gel was used. The mixed solution of acetonitrile/50mM KH2PO4 (adjusted to pH 2.5 with 0.001 volumes of 85 g/dl H3PO4)(20/80) was a favorable mobile phase for the separation of ALA. Amino acetone pyrrole was produced by the condensation of amino acetone with methyl acetoacetate. The amino acetone pyrrole appeared later than ALA pyrrole on HPLC. The method is simple and specific. It has a lower detection limit of 0.2 ng on column. The analysis and quantitative determination of one specimen can be performed within 20 min. Mean ALA concentration of normal urine by HPLC was 1.18mg/g creatinine.
Key wordsDelta-aminolevulinic acid ALA pyrrole Lead exposure High performance liquid chromatography
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- Cramer K, Selander S (1965) Studies in lead poisoning. Comparison between different laboratory tests. Br J Ind Med 22:311–314Google Scholar
- Mauzerall D, GranickS (1956) The occurrence and determination of delta-aminolevulinic acid and porphobilinogen in urine. J Biol Chem 219:435–446Google Scholar
- Taguchi T, Suzuki S, Yokohashi G (1970) Normal levels of lead, delta-aminolevulinic acid and coproporphyrin spot urines. Jpn J Ind Health 12:18–19 (in Japanese)Google Scholar
- Tomokuni K, Ogata M (1972) Simple method for determination of urinary delta-aminolevulinic acid as an index exposure. Clin Chem 18:1534–1536Google Scholar
- Tomokuni K, Ogata M (1980) Normal levels of delta-aminolevulinic acid in the spot urines of adult men, as measured by a simplified method. Jpn J Ind Health 22:92–95 (in Japanese)Google Scholar
- Tomokuni K, Hasegawa N, Sugawara E (1986) Improved method for the determination of urinary delta-aminolevulinic acid in lead workers. Jpn J Ind Health 28:114–118Google Scholar
- Urata G, Granick S (1963) Biosynthesis of α-aminoketones and the metabolism of aminoacetone. J Biol Chem 238:811–820Google Scholar
- Wada O, Toyokawa K, Urata G, Yano Y, Nakao K (1969) A simple method for the quantitave analysis of urinary delta-aminolevulinic acid to evaluate lead absorption. Br J Ind Med 26:240–243Google Scholar