Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Isolation and characterization of xylose- and xylan-utilizing anaerobic bacteria

  • 119 Accesses

  • 11 Citations


By enrichment with xylose, nine mesophilic strains of anaerobic bacteria were obtained from various sources. Two isolates appear to belong to the genus Eubacterium. Six other strains belong to the genus Clostridium. Three of the isolated strains utilized larch wood xylan. The percentage of utilization of xylose and xylan and the yield of fermentation end products — viz. acetic acid and butyric acid-are equivalent to that of Clostridium acetobutylicum (ATCC 824) and reported thermophilic strains.

This is a preview of subscription content, log in to check access.


  1. Buchanan RE & Gibbons NE (Eds) (1974) Bergey's Manual of Determinative Bacteriology. 8th edn. The Williams and Wilkins Co., Baltimore

  2. Crawford DL (1978) Lignocellulose decomposition by selected Streptomyces strains, Appl. Environ. Microbiol 35: 1041–1045

  3. Dubois M, Gilles KA, Hamilton JK, Rebers P & Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356

  4. Henning PA (1979) Examination of methods for enumerating hemicellulose-utilizing bacteria in the rumen. Appl. Environ. Microbiol. 38: 13–17

  5. Holdeman LV, Cato EP & Moore WEC (Eds) (1977) Anaerobic Laboratory Manual. 4th edn. Anaerobic Laboratory, Virginia Polytechnic Institute and State University, Blacksburg

  6. Jeffries TW (1983) Utilization of xylose by bacteria, yeasts and fungi. Adv. Biochem. Eng. Biotechnol. 27: 1–32

  7. Lemmel SA, Datta R & Frankiewicz JR (1986) Fermentation of xylan by Clostridium acetobutylicum. Enzyme Microb. Technol. 8: 217–221

  8. Le Ruyet P, Dubourguier HC & Albagnac G (1984) Thermophilic fermentation of cellulose and xylan by methanogenic enrichment cultures: preliminary characterization of main species. System Appl. Microbiol. 5: 247–253

  9. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275

  10. Miller TL & Wolin MJ (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl. Microbiol. 27: 985–987

  11. Müller A (1971) Analytical chemistry. In: Holten CH (Ed) Lactic Acid. Properties and Chemistry of Lactic Acid and Derivatives (pp 461–487) Verlag Chemie

  12. Ounine K, Petitdemange H, Raval G & Gay R (1983) Acetone-butanol production from pentoses by Clostridium acetobutylicum. Biotechnol. Lett. 5: 605–610

  13. Patel GB (1984) Ethanol production during D-xylose, L-arabinose and D-ribose fermentation by Bacteroides polypragmatus. Appl. Microbiol. Biotechnol. 20: 111–117

  14. Sleat R & Mah RA (1984) Quantitative method for colorimetric determination of formate in fermentation media. Appl. Environ. Microbiol. 47: 884–885

  15. Timell TE & Syracuse NY (1967) Recent progress in chemistry of wood hemicelluloses. Wood Sci. Technol. 1: 45–70

  16. Van der Toorn JJTK & Van Gylswyk NO (1985) Xylan-digesting bacteria from the rumen of sheep fed maize straw diets. J. Gen. Microbiol. 131: 2601–2607

  17. Wilkie KCB (1976) The hemicelluloses of grasses and cereals. In: Tipson S & Horton D (Eds) Advances in Carbohydrate Chemistry and Biochemistry. Vol. 36 (pp 215–264) Academic Press, New York

  18. Zeikus JG (1980) Chemical and Fuel production by anaerobic bacteria. Annu. Rev. Microbiol. 34: 423–464

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Murty, M.V.S., Chandra, T.S. Isolation and characterization of xylose- and xylan-utilizing anaerobic bacteria. Antonie van Leeuwenhoek 55, 153–163 (1989). https://doi.org/10.1007/BF00404755

Download citation

Key words

  • anaerobes
  • fermentation
  • hemicellulose
  • xylan
  • xylose