By enrichment with xylose, nine mesophilic strains of anaerobic bacteria were obtained from various sources. Two isolates appear to belong to the genus Eubacterium. Six other strains belong to the genus Clostridium. Three of the isolated strains utilized larch wood xylan. The percentage of utilization of xylose and xylan and the yield of fermentation end products — viz. acetic acid and butyric acid-are equivalent to that of Clostridium acetobutylicum (ATCC 824) and reported thermophilic strains.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Buchanan RE & Gibbons NE (Eds) (1974) Bergey's Manual of Determinative Bacteriology. 8th edn. The Williams and Wilkins Co., Baltimore
Crawford DL (1978) Lignocellulose decomposition by selected Streptomyces strains, Appl. Environ. Microbiol 35: 1041–1045
Dubois M, Gilles KA, Hamilton JK, Rebers P & Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356
Henning PA (1979) Examination of methods for enumerating hemicellulose-utilizing bacteria in the rumen. Appl. Environ. Microbiol. 38: 13–17
Holdeman LV, Cato EP & Moore WEC (Eds) (1977) Anaerobic Laboratory Manual. 4th edn. Anaerobic Laboratory, Virginia Polytechnic Institute and State University, Blacksburg
Jeffries TW (1983) Utilization of xylose by bacteria, yeasts and fungi. Adv. Biochem. Eng. Biotechnol. 27: 1–32
Lemmel SA, Datta R & Frankiewicz JR (1986) Fermentation of xylan by Clostridium acetobutylicum. Enzyme Microb. Technol. 8: 217–221
Le Ruyet P, Dubourguier HC & Albagnac G (1984) Thermophilic fermentation of cellulose and xylan by methanogenic enrichment cultures: preliminary characterization of main species. System Appl. Microbiol. 5: 247–253
Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275
Miller TL & Wolin MJ (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl. Microbiol. 27: 985–987
Müller A (1971) Analytical chemistry. In: Holten CH (Ed) Lactic Acid. Properties and Chemistry of Lactic Acid and Derivatives (pp 461–487) Verlag Chemie
Ounine K, Petitdemange H, Raval G & Gay R (1983) Acetone-butanol production from pentoses by Clostridium acetobutylicum. Biotechnol. Lett. 5: 605–610
Patel GB (1984) Ethanol production during D-xylose, L-arabinose and D-ribose fermentation by Bacteroides polypragmatus. Appl. Microbiol. Biotechnol. 20: 111–117
Sleat R & Mah RA (1984) Quantitative method for colorimetric determination of formate in fermentation media. Appl. Environ. Microbiol. 47: 884–885
Timell TE & Syracuse NY (1967) Recent progress in chemistry of wood hemicelluloses. Wood Sci. Technol. 1: 45–70
Van der Toorn JJTK & Van Gylswyk NO (1985) Xylan-digesting bacteria from the rumen of sheep fed maize straw diets. J. Gen. Microbiol. 131: 2601–2607
Wilkie KCB (1976) The hemicelluloses of grasses and cereals. In: Tipson S & Horton D (Eds) Advances in Carbohydrate Chemistry and Biochemistry. Vol. 36 (pp 215–264) Academic Press, New York
Zeikus JG (1980) Chemical and Fuel production by anaerobic bacteria. Annu. Rev. Microbiol. 34: 423–464
About this article
Cite this article
Murty, M.V.S., Chandra, T.S. Isolation and characterization of xylose- and xylan-utilizing anaerobic bacteria. Antonie van Leeuwenhoek 55, 153–163 (1989). https://doi.org/10.1007/BF00404755