Immunogenetics

, Volume 22, Issue 3, pp 277–283 | Cite as

Localization of CTβ and C K on mouse chromosome 6

  • Lawrence A. D'Hoostelaere
  • Evelyne Jouvin-Marche
  • Konrad Huppi
Article
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Abstract

In the mouse three lymphocyte gene families have been positioned on the proximal region of chromosome 6. Originally the immunoglobulin kappa light chain (Igk) and the thymocyte surface antigens Lyt-2 and Lyt-3 were assigned to chromosome 6, and recently the beta chain of the T-cell receptor for antigen was positioned proximal to Igk. Molecular clones which recognize the constant (C region of the beta chain of the T-cell receptor for antigen (CTβ) and the constant region of the immunoglobulin kappa (C k ) chain were used to determine recombination frequencies with respect to the morphological marker hypodactyly (Hd). SJL/JLw π mice were mated with C.B6.C3-Hd/+ mice, and the progeny expressing the Hd phenotype were mated with SJL/JLw π mice. Backcross progeny which expressed the Hd phenotype were nephrectomized, and kidney DNA was examined by Southern hybridization for the polymorphic restriction endonuclease fragment (REF) patterns of the parental mice. Of the 88 progeny tested in this three-point cross, 3 CTβ and 4 C K homozygote REF patterns were detected. These homozygotes were mutually exclusive. This implies the following gene order: centromere-C Tβ -Hd-Igk and C Tβ1 would be 7.95±2.88 centimorgans from C K .

Keywords

Gene Order Southern Hybridization Proximal Region Recombination Frequency Mouse Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Lawrence A. D'Hoostelaere
    • 1
  • Evelyne Jouvin-Marche
    • 1
  • Konrad Huppi
    • 1
  1. 1.Laboratory of Genetics, National Cancer InstituteNational Institutes of HealthBethesda

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