Antibodies to orthokeratotic keratinocytes in monitoring the drug-induced inhibition of parakeratotic differentiation in adult and infant mice
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Parakeratosis (PK) is a common feature of the abnormal epidermis in several disorders of keratinization. Tar phenols, retinoids and steroids which cause the inhibition of PK and the restoration of orthokeratosis (OK) are used for treating psoriasiform conditions. In this work, we studied two experimental models of the drug-induced inhibition of PK:(1) the suppression of the normal development of PK in the infant mouse tail and (2) the OK conversion of established PK in the adult tail. Two markers of OK were studied: the histological evaluation of the granular layer and the expression of cytoplasmic antigens linked to OK differentiation. It was demonstrated that high-boiling tar phenols cause a more potent inhibition of PK than betamethasone valerate. Most importantly, immunofluorescence showed that the switch to OK differentiation was located in the cells situated in a suprabasal position. The use of these immunological markers to investigate and anti-parakeratotic mechanisms has revealed that these drugs act at stages of keratinocyte differentiation which are distinct from those previously suggested.
Key wordsMouse tail Parakeratosis Antiparakeratotic drugs Keratinization Orthokeratotic antigens
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- 5.Galoppin L, Didierjean L, Saurat J-H (1981) Etude de la fixation des anticorps anti-cytoplasme des keratinocytes. Discussion du rôle du fragment Fc. Ann Dermatol Venereol 108:201Google Scholar
- 6.Jarrett A, Spearman RIC (1964) Histochemistry of the skin — psoriasis. English University Press, LondonGoogle Scholar
- 9.Römer F (1896) Studien über das Integument der Säugetiere. I. Die Entwicklung der Schuppen und Haare am Schwanze und an den Füssen von Mus decumanus und einigen anderen Muriden. Jena Z Med Naturwissenschaft 30:604–622Google Scholar
- 11.Saurat J-H, Didierjean L, Galoppin L, Gluckman E (1978) Antibodies with affinity to the cytoplasm of keratinocytes produced after bone marrow transplantation in man. A review. Cut Immunopathol INSERM (Paris) 80:77–92Google Scholar
- 16.Spearman RIC (1964) The evolution of mammalian keratinized structures. In: Ebling J (ed) The mammalian epidermis and its derivatives. Symposium of the Zoological Society London no. 12. Academic Press, London, pp 67–81Google Scholar
- 17.Wrench R (1977) Changes in mouse tail epidermal keratinization induced by tar derivatives. In: Spearman RIC (ed) Comparitive biology of skin. Symposium of the Zoological Society London no. 39. Academic Press, London, pp 353–372Google Scholar