Springer Nature is making SARS-CoV-2 and COVID-19 research free View research | View latest news | Sign up for updates

Insulin-driven erythropoiesis may underlie impairment of erythrocyte deformability in hyperinsulinaemic, hyperglycaemic ob/ob-mice

  • 64 Accesses

  • 7 Citations

Summary

The circulating erythrocytes in hyperglycaemic and hyperinsulinaemic obese (ob/ob) mice are enlarged with a decreased erythrocyte filtrability and an abnormally low resistance to osmotic stress. These changes probably reflect aberrations of erythropoiesis, as evidenced by enhanced staining for iron in the bone marrow, reticulocytosis, and increased erythrocyte volume fraction. Mature erythrocytes, reticulocytes, and late-phase basophilic erythroblasts were found to have larger diameters than their counterparts in control mice while myelopoiesis appeared to be unaffected. The average erythrocyte also displayed an increased cell volume and a decreased haemoglobin concentration. It is suggested that the stimulation of the erythroid cell line in ob/ob-mice might be a consequence of the hereditary hyperinsulinaemia.

References

  1. 1.

    Chien S, Usami S, Dellenback RJ, Gregersen MI (1967) Blood viscosity: influence of erythrocyte deformation. Science 157: 827–829

  2. 2.

    McMillan DE, Utterback NG, La Puma J (1978) Reduced erythrocyte deformability in diabetes. Diabetes 27: 895–901

  3. 3.

    Schmid-Schönbein H, Volger E (1976) Red-cell aggregation and red-cell deformability in diabetes. Diabetes 25 [Suppl 2]: 897–902

  4. 4.

    Ernst E, Matrai A (1986) Altered red and white blood cell rheology in type II diabetes. Diabetes 35: 1412–1415

  5. 5.

    Engström KG, Täljedal I-B (1986) Decreased deformability of erythrocytes in hyperglycaemic non-inbred ob/ob-mice. Diabetologia 29: 661–666

  6. 6.

    Engström KG (1989) A new red blood cell filtration device with improved time resolution and its application to the impaired RBC deformability in the diabetic ob/ob mouse. Biorheology 26: 711–721

  7. 7.

    Volger E (1981) Effect of metabolic control and concomitant diseases upon the rheology of blood in different states of diabetic retinopathy. Horm Metab Res [Suppl 11]: 104–107

  8. 8.

    McMillan DE, Utterback NG, Romanoff NE, Mahler RJ (1986) Transient blood flow resistance is markedly increased in diabetes. Diabetologia 29: 575, (Abstract)

  9. 9.

    Engström KG, Täljedal I-B (1987) Altered shape and size of red blood cells in obese hyperglycaemic mice. Acta Physiol Scand 130: 535–543

  10. 10.

    Engström KG, Täljedal I-B (1988) Increased size and biconcavity in red blood cells of obesehyperglycaemic mice. Biorheology 25: 625–638

  11. 11.

    Westman S (1968) Development of the obesehyperglycaemic syndrome in mice. Diabetologia 4: 141–149

  12. 12.

    Kurtz A, Jelkmann W, Bauer C (1983) Insulin stimulates erythroid colony formation independently of erythropoietin. Br J Haematol 53: 311–316

  13. 13.

    Dainiak N, Kreczko S (1985) Interactions of insulin, insulin-like growth factor II, and platelet-derived growth factor in erythropoietic culture. J Clin Invest 76: 1237–1242

  14. 14.

    Täljedal I-B (1981) On insulin secretion. Diabetologia 21: 1–17

  15. 15.

    Nathorst-Windahl G, Hellman B (1964) Lipohyalin glomerular lesions in ageing obese-hyperglycemic mice. Med Exp 10: 67–71

  16. 16.

    Bergstrand A, Nathorst-Windahl G, Hellman B (1968) The electron microscopic appearance of the glomerular lesions in obesehyperglycaemic mice. Acta Pathol Microbiol Scand 74: 161–168

  17. 17.

    Bohlen HG, Niggl BA (1979) Arteriolar anatomical and functional abnormalities in juvenile mice with genetic or streptozotocin-induced diabetes mellitus. Circ Res 45: 390–396

  18. 18.

    Collison AH, Rodney FL, O'Neel J (1967) Determination of carbon monoxide in blood by gas chromatography. Clin Chem 14: 161–171

  19. 19.

    Dacie JV, Lewis SM (1975) Practical haematology, 5th edn. Churchill Livingstone, Edinburgh

  20. 20.

    Russell ES, Bernstein SE (1966) Blood and blood formation. In: Green EL (ed) Biology of the laboratory mouse. McGraw-Hill, New York, pp 351–372

  21. 21.

    Leblond PF, Coulombe L (1979) The measurement of erythrocyte deformability using micropore membranes. J Lab Clin Med 94: 133–143

  22. 22.

    Nash GB, Wyard SJ (1980) Changes in surface area and volume measured by micropipette aspiration for erythrocytes ageing in vivo. Biorheology 17: 479–484

  23. 23.

    Linderkamp O, Meiselman HJ (1982) Geometric, osmotic, and membrane mechanical properties of density-separated human red cells. Blood 59: 1121–1127

  24. 24.

    Hollenberg MD, Cuatrecasas P (1975) Insulin and epidermal growth factor: human fibroblast receptors related to deoxyribonucleic acid synthesis and amino acid uptake. J Biol Chem 250: 3848–3853

  25. 25.

    Mullis P, Schuler J, Zuppinger K (1989) Increased prevalence of fetal haemoglobin in Type 1 (insulin-dependent) diabetes mellitus. Diabetologia 32: 227–230

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Engström, K.G., Grankvist, K. & Täljedal, I.-. Insulin-driven erythropoiesis may underlie impairment of erythrocyte deformability in hyperinsulinaemic, hyperglycaemic ob/ob-mice. Diabetologia 33, 127–130 (1990). https://doi.org/10.1007/BF00404037

Download citation

Key words

  • Erythrocyte
  • erythropoiesis
  • insulin
  • erythrocyte deformability
  • erythrocyte shape
  • ob/ob-mice