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Diabetologia

, 39:946 | Cite as

The role of glycation cross-links in diabetic vascular stiffening

  • T. J. Sims
  • L. M. Rasmussen
  • H. Oxlund
  • A. J. Bailey
Originals

Summary

Previous studies have shown that biomechanical analysis of aorta from diabetic subjects reveals a marked increase in stiffness compared to aorta from age-matched control subjects. In the present paper we have proposed that this increased stiffness can be attributed to glycation-induced inter-molecular cross-links based on a direct analysis of the two known glycation cross-links, the fluorescent pentosidine and the non-fluorescent NFC-1. There was a significant difference in the increase in concentration of both cross-links with increasing age for both the intima (p<0.0025) and the media (p<0.0005) from the diabetic compared to the control subjects, but no correlation with the mature enzymic cross-link hydroxylysyl-pyridinoline. Finally, we have obtained a significant correlation of stiffness with both glycation cross-links (NFC-1, r=0.86; p<0.005 and pentosidine r=0.75, p<0.05), but the concentration of NFC-1 is about 50 times greater than that of pentosidine, indicating that it is the major glycation cross-link responsible for the stiffening of the aorta.

Keywords

Diabetes mellitus glycation cross-links vascular stiffening 

Abbreviations

AGE

Advanced glycation end-product

FFI

furoyl-furanyl imidazole

HHL

histidino-hydroxylysinonor-leucine

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • T. J. Sims
    • 1
  • L. M. Rasmussen
    • 2
  • H. Oxlund
    • 2
  • A. J. Bailey
    • 1
  1. 1.Collagen Research Group, Division of Molecular and Cellular BiologyUniversity of BristolLangfordUK
  2. 2.Department of Connective Tissue Biology, Institute of AnatomyUniversity of AarhusAarhusDenmark

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