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Photoinhibition at chilling temperature

Fluorescence characteristics of unhardened and cold-acclimated spinach leaves

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Abstract

The effects of moderate light at chilling temperature on the photosynthesis of unhardened (acclimated to +18° C) and hardened (cold-acclimated) spinach (Spinacea oleracea L.) leaves were studied by means of fluorescence-induction measurements at 20° C and 77K and by determination of quantum yield of O2 evolution. Exposure to 550 μmol photons·m-2·s-1 at +4° C induced a strong photoinhibition in the unhardened leaves within a few hours. Photoinhibition manifested by a decline in quantum yield was characterized by an increase in initial fluorescence (F o) and a decrease in variable fluorescence (F v) and in the ratio of variable to maximum fluorescence (F V/F M), both at 77K and 20° C. The decline in quantum yield was more closely related to the decrease in the F V/F M ratio measured at 20° C, as compared with F V/F M at 77K. Quenching of the variable fluorescence of photosystem II was accompanied by a decline in photosystem-I fluorescence at 77K, indicating increased thermal de-excitation of pigments as the main consequence of the light treatment. All these changes detected in fluorescence parameters as well as in the quantum yield of O2 evolution were fully reversible within 1–3 h at a higher temperature in low light. The fast recovery led us to the view that this photoinhibition represents a regulatory mechanism protecting the photosynthetic apparatus from the adverse effects of excess light by increasing thermal energy dissipation. Long-term cold acclimation probably enforces other protective mechanisms, as the hardened leaves were insensitive to the same light treatment that induced strong inhibition of photosynthesis in unhardened leaves.

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Abbreviations

F 0 :

initial fluorescence

F M :

maximum fluorescence

F V :

variable fluorescence (F M-F 0

PFD:

photon flux density

PS:

photosystem

References

  1. Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15

  2. Baker, N.R., Horton, P. (1987) Chlorophyll fluorescence quenching during photoinhibition. In: Photoinhibition, topics of photosynthesis, vol. 9, pp. 145–168, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam

  3. Barényi, B., Krause, G.H. (1985) Inhibition of photosynthetic reactions by light. A study with isolated spinach chloroplasts. Planta 163, 218–226

  4. Björkman, O. (1987) Low-temperature chlorophyll fluorescence in leaves and its relationship to photon yield in photoinhibition. In: Photoinhibition, topics of photosynthesis, vol. 9, pp. 123–144, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam

  5. Björkman, O., Demmig, B. (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origins. Planta 170, 489–504

  6. Bradbury, M., Baker, N.R. (1986) The kinetics of photoinhibition of the photosynthetic apparatus in pea chloroplasts. Plant Cell Environ. 9, 289–297

  7. Butler, W.L. (1978) Energy distribution in the photochemical apparatus of photosynthesis. Annu. Rev. Plant Physiol. 29, 345–378

  8. Chow, W.S., Osmond, C.B., Huang, L.K. (1988) Photosystem II function and herbicide binding sites during photoinhibition of spinach chloroplasts in-vivo and in-vitro. Photosynth. Res., in press

  9. Cleland, R.E., Melis, A., Neale, P.J. (1986) Mechanism of photoinhibition: photochemical reaction center inactivation in system II of chloroplasts. Photosynth. Res. 9, 79–88

  10. Demmig, B., Björkman, O. (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution of leaves of higher plants. Planta 171, 171–184

  11. Greer, D.H., Berry, J.A., Björkman, O. (1986) Photoinhibition of photosynthesis in intact bean leaves: a role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta 168, 253–260

  12. Horton, P., Hague, A. (1988) Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV. Resolution of non-photochemical quenching. Biochim. Biophys. Acta 932, 107–115

  13. Kitajima, M., Butler, W.L. (1975) Excitation spectra for photosystem II in chloroplasts and the spectral characteristics of the distribution of quanta between the two photosystems. Biochim. Biophys. Acta 408, 297–305

  14. Klosson, R.J., Krause, G.H. (1981) Freezing injury in cold acclimated and unhardened spinach leaves. I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves. Planta 151, 339–346

  15. Krause, G.H., Behrend, U. (1986) ΔpH-dependent chlorophyll fluorescence quenching indicating a mechanism of protection against photoinhibition of chloroplasts. FEBS Lett. 200, 298–302

  16. Krause, G.H., Briantais, J.-M., Vernotte, C. (1983) Characterization of chlorophyll fluorescence quenching in chloroplasts by fluorescence spectroscopy at 77K. ΔpH-dependent quenching. Biochim. Biophys. Acta 723, 169–175

  17. Krause, G.H., Köster, S., Wong, S.C. (1985) Photoinhibition of photosynthesis under anaerobic conditions studied with leaves and chloroplasts of Spinacia oleracea L. Planta 165, 430–438

  18. Krause, G.H., Laasch, H. (1987) Energy-dependent chlorophyll fluorescence quenching in chloroplasts correlated with quantum yield of photosynthesis. Z. Naturforsch. 42c, 581–584

  19. Kyle, D.J. (1987) The biochemical basis for photoinhibition of photosystem II. In: Photoinhibition, topics of photosynthesis, vol. 9, pp. 197–226, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam

  20. Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. (1987) Photoinhibition, topics in photosynthesis, vol. 9. Elsevier, Amsterdam

  21. Leverenz, J.W., Öquist, G. (1987) Quantum yields of photosynthesis at temperatures between — 2° C and 35° C in the course of one year. Plant Cell Environ. 10, 287–295

  22. Mäenpää, P., Andersson, B., Sundby, C. (1987) Difference in sensitivity to photoinhibition between photosystem II in appressed and non-appressed thylakoid regions. FEBS Lett. 215, 31–36

  23. Mäenpää, P., Aro, E.-M., Somersalo, S., Tyystjärvi, E. (1988) Rearrangement of the chloroplast thylakoid at chilling temperature in the light. Plant Physiol. 87, 762–766

  24. Ögren, E., Öquist, G. (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. III. Chlorophyll fluorescence at 77K. Physiol. Plant. 62, 193–200

  25. Öquist, G., Greer, D.H., Ögren, E. (1987) Light stress at low temperatures. In: Photoinhibition, topics of photosynthesis, vol. 9, pp. 67–87, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam

  26. Powles, S.B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol. 35, 15–44

  27. Powles, S.B., Berry, J.A., Björkman, O. (1983) Interaction between light and chilling temperature on the inhibition of photosynthesis in chilling-sensitive plants. Plant Cell Eviron. 6, 117–123

  28. Powles, S.B., Björkman, O. (1982) Photoinhibition of photosynthesis: effect on chlorophyll fluorescence at 77K in intact leaves and in chloroplast membranes of Nerium oleander. Planta 156, 97–107

  29. Samuelsson, G., Lönneborg, A., Gustavsson, P., Öquist, G. (1987) The susceptibility of photosynthesis to photoinhibition and the capacity of recovery in high and low light grown cyanobacteria, Anacystis nidulans. Plant Physiol. 83,438–441

  30. Sundby, C., Melis, A., Mäenpää, P., Andersson, B. (1986) Temperature-dependent changes in the antenna size of photosystem II. Reversible conversion of photosystem IIα to photosystem II. Biochim. Biophys. Acta 851, 475–483

  31. Weis, E., Berry, J.A. (1987) Quantum efficiency of photosystem II in relation to ‘energy’-dependent quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 894, 198–208

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Correspondence to G. H. Krause.

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Somersalo, S., Krause, G.H. Photoinhibition at chilling temperature. Planta 177, 409–416 (1989). https://doi.org/10.1007/BF00403600

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Key words

  • Chlorophyll fluorescence
  • Cold acclimation
  • Photoinhibition
  • Photosynthesis (inhibition)
  • Quantum yield
  • Spinacia (cold acclimation)