Planta

, Volume 177, Issue 3, pp 281–295

Osmotic responses of maize roots

Water and solute relations
  • Ernst Steudle
  • Jürgen Frensch
Article

DOI: 10.1007/BF00403585

Cite this article as:
Steudle, E. & Frensch, J. Planta (1989) 177: 281. doi:10.1007/BF00403585

Abstract

Water and solute relations of excised seminal roots of young maize (Zea mays L) plants, have been measured using the root pressure probe. Upon addition of osmotic solutes to the root medium, biphasic root pressure relaxations were obtained as theoretically expected. The relaxations yielded the hydraulic conductivity Lpr) the permeability coefficient (Psr), and the reflection coefficient (σsr) of the root. Values of Lpr in these experiments were by nearly an order of magnitude smaller than Lpr values obtained from experiments where hydrostatic pressure gradients were used to induce water flows. The value of Psr was determined for nine different osmotica (electrolytes and nonelectrolytes) which resulted in rather variable values (0.1·10-8–1.7·10-8m·s-1). The reflection coefficient σsr of the same solutes ranged between 0.3 and 0.6, i.e. σsr was low even for solutes for which cell membranes exhibit a σs≈1. Deviations from the theoretically expected biphasic responses occured which may have reflected changes of either Psr or of active pumping induced by the osmotic change. The absolute values of Lpr, Psr, and σsr have been critically examined for an underestimation by unstirred layer effecs. The data indicate a considerable apoplasmic component for radial movement of water in the presence of hydrostatic gradients and also some solute flow byppassing root protoplasts. In the presence of osmotic gradients, however, there was a substantial cell-to-cell transport of water. Cutting experiments demonstrated that the hydraulic resistance for the longitudinal movement of water was much smaller than for radial transport except for the apical ends of the segments (length=5 to 20 mm). The differences in Lpr as well as the low σsr values suggest that the simple osmometer model of the root with a single osmotic barrier exhibiting nearly semipermeable properties should be extended for a composite membrane model with hydraulic and osmotic barriers arranged in series and in parallel.

Key words

Hydraulic conductivity Reflection coefficient Root permeability Root pressure Water relations Zea (water relations) 

Abbreviations and symbols

Ar

root surface area

Cm (CX)

solute concentration in the meduum (xylem)

DSS(DSC)

diffusion coefficient in the stele (cortex)

JVr

radia water transport across the root

kwr (ksr)

rate constant of water (solute exchange between root xylem and medium

Lpr

root hydroaulic conductivity

Pr

root pressure

Psr

permeability coefficient of root for solute “s”

Tr12/S(Tr12/S)

half-time of water (solute) exchange between root xylem and medium

VS (Vx)

volume of system (xylem)

δs(δc)

thickness of unstirred layer in the stele (cortex)

εSεX

elastic modulus of measuring system (xylem)

σsr

reflection coefficient of root for solute “s”

Φ

flow constriction factor; superscripts “en” and “ex” denote flows from the medium into the root xylem or from the xylem into the medium, respectively

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Ernst Steudle
    • 1
  • Jürgen Frensch
    • 1
  1. 1.Lehrstuhl für PflanzenökologieUniversität BayreuthBayreuthFederal Republic of Germany

Personalised recommendations