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Photooxidase activity of isolated chromatophores and intact cells of phototrophic bacteria

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Illumination causes an uptake of oxygen by isolated chromatophores of purple and green bacteria incubated with electron donors. Photooxidase activity of Rhodospirillum rubrum, Chromatium minutissimum, Rhodopseudomonas sphaeroides and Thiocapsa roseopersicina chromatophores is sensitive, and photooxidase activity of Ectothiorhodospira shaposhnikovii and Chlorobium limicola f. thiosulfatophilum is resistant to o-phenanthroline. O2 uptake by illuminated chromatophores of R. rubrum and C. limicola is stimulated upon the increase of pH of incubation mixture from 5 to 9. Photooxidase activity is also manifested in the intact bacterial cells and not merely in the isolated chromatophores. O2 uptake by the illuminated R. rubrum cells treated with CN- is stimulated by 2-heptyl-4-hydroxyquinoline-N-oxide and a protonophorous uncoupler. The interaction of the photosynthetic and respiratory systems of the electron transfer in the bacterial cells and the probable causes of the strong anaerobic way of life of the green sulfur bacteria are discussed.

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  1. Asami, S., Akazawa, T.: Enzymic formation of glycolate in Chromatium. Role of superoxide radical in a transketolase-type mechanism. Biochemistry 16, 2202–2207 (1977)

  2. Baccarini-Mclandri, A., Jones, O. T. G., Hauska, G.: Cytochrome c 2 — an electron carrier shared by the respiratory and photosynthetic electron transport chain of Rhodopseudomonas capsulata. FEBS Lett. 86, 151–154 (1978)

  3. Barouch, Y., Clayton, R. K.: Ubiquinone reduction and proton uptake by chromatophores of Rhodopseudomonas sphaeroides R-26. Periodicity of two in consecutive light flashes. Biochim. Biophys. Acta 462, 785–788 (1977)

  4. Bogorov, L. V.: Properties of Thiocapsa roseopersicina strain BBS isolated from the estuary of the White Sea. Microbiologiya 33, 326–332 (1974)

  5. Borisov, A. Yu., Kondratieva, E. N., Samuilov, V. D., Skulachev, V. P.: The interrelation between the NAD photoreduction and phosphorylation processes in chromatophores of Rhodospirillum rubrum. Mol. Biologiya 4, 795–807 (1970)

  6. Bose, S. K., Gest, H., Ormerod, J. G.: Light-activated hydrogenase activity in a photosynthetic bacterium: a permeability phenomenon. J. biol. Chem. 236, PC 13–14 (1961)

  7. Boucher, F., Gingras, G.: The photogeneration of superoxide by isolated photoreaction center from Rhodospirillum rubrum. Biochem. Biophys. Res. Commun. 67, 421–426 (1975)

  8. Clayton, R. K.: Competition between light and dark metabolism in Rhodospirillum rubrum. Arch. Mikrobiol. 22, 195–203 (1955)

  9. Clayton, R. K.: Absorption spectra of photosynthetic bacteria and their chlorophylls. In: Bacterial Photosynthesis (H. Gest, A. San Pietro, L. P. Vernon, eds.), pp. 495–500. Yellow Springs, Ohio: Antioch Press 1963

  10. Cogdell, R. J., Brune, D. C., Clayton, R. K.: Effects of extraction and replacement of ubiquinone upon the photochemical activity of reaction centers and chromatophores from Rhodopseudomonas spheroides. FEBS Lett. 45, 344–347 (1974)

  11. del Valle-Tascon, S., Ramirez, J. M.: Origin of the ATP formed during the light-dependent oxygen uptake catalyzed by Rhodospirillum rubrum chromatophores. Z. Naturforsch. 30 c, 46–52 (1975)

  12. Dutton, P. L., Prince, R. C., Tiede, D. M., Petty, K. M., Kaufmann, K. J., Netzel, T. L., Rentzepis, P. M.: Electron transfer in the photosynthetic reaction center. Brookhaven Symp. Biol., no. 28 (J. M. Olson, G. Hind, eds.), pp. 183–194. New York: Upton 1977

  13. Feldman, N., Gromet-Elhanan, Z.: Non-cyclic electron transport and photophosphorylation in Rhodospirillum rubrum chromatophores. Proceedings of the 2nd Internat. Congress on Photosynthesis Res., vol. 2, pp. 1211–1220. (G. Forti, M. Avron, A. Melandri, eds.). The Hague, N. V. Publishers (1972)

  14. Geller, D. M.: Oxidative phosphorylation in extracts of Rhodospirillum rubrum. J. biol. Chem. 237, 2947–2954 (1962)

  15. Gusev, M. V., Shenderova, L. V., Kondratieva, E. N.: The effect of molecular oxygen on various species of photosynthetizing bacteria. Microbiologiya 38, 787–792 (1969)

  16. Hochman, A., Ben-Hayyim, G., Carmeli, C.: Light-induced electron transport pathways in membrane preparations from Rhodopseudomonas capsulata. Arch. Biochem. Biophys. 184, 416–422 (1977)

  17. Hochman, A., Carmeli, C.: Reconstitution of photosynthetic electron transport and photophosphorylation in cytochrome-c 2-deficient membrane preparation of Rhodopseudomonas capsulata. Arch. Biochem. Biophys. 179, 349–359 (1977)

  18. Kakuno, T., Bartsch, R. G., Nishikawa, K., Horio, T.: Redox components associated with chromatophores from Rhodospirillum rubrum. J. Biochem. 70, 79–91 (1971)

  19. Keister, D. L., Minton, N. J.: Effect of light on respiration in Rhodospirillum rubrum chromatophores. In: Energy transduction in respiration and photosynthesis (E. Quagliariello, S. Papa, C. S. Rossi, eds.), pp. 375–384. Pari: Adriatica Editrice 1971

  20. Kondratieva, E. N.: Photosynthetic Bacteria. Moscow: U.S.S.R. Sci. Acad. Press 1963

  21. Larsen, H.: On the microbiology and biochemistry of the photosynthetic green sulfur bacteria. K. Norske Vidensk. Selskabs Skr. 1, 1–205 (1953)

  22. McCord, J. M., Fridovich, I.: Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. biol. Chem. 244, 6049–6055 (1969)

  23. Nakamura, H.: Über die Photosynthese bei der schwefelfreien Purpurbakterie, Rhodobacillus palustris. Beiträge zur Stoffwechselphysiologie der Purpurbakterien. Acta Phytochim. 9, 189–229 (1937)

  24. Okamura, M. Y., Isaacson, R. A., Feher, C.: Primary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of Rhodopseudomonas sphaeroides. Proc. Natl. Acad. Sci. USA 72, 3491–3495 (1975)

  25. Olson, J. M., Prince, R. C., Brune, D. C.: Reaction-center complexes from green bacteria. Brookhaven Symp. Biol., no. 28 (J. M. Olson, G. Hind, eds.), pp. 238–246. New York: Upton 1977

  26. Remennikov, V. G., Samuilov, V. D.: Light-induced oxygen uptake by chromatophores and subchromatophore pigment-protein complexes of Rhodospirillum rubrum. Biokhimiya 42, 1997–2004 (1977)

  27. Remennikov, V. G., Samuilov, V. D.: Photooxidase activity of Rhodospirillum rubrum chromatophores and reaction center complexes. The role of non-cyclic electron transfer in the membrane potential generation. Biochim. Biophys. Acta 546, 220–235 (1979)

  28. Samuilov, V. D., Kondratieva, E. N.: A study of photophosphorylation in chromatophores from different bacterial species. Biol. Nauki 5, 97–100 (1969)

  29. Sasaki, T., Motokawa, Y., Kikuchi, G.: Occurence of both a-type and o-type cytochromes as the functional terminal oxidases in Rhodopseudomonas spheroides. Biochim. Biophys. Acta 197, 284–291 (1970)

  30. Smith, L., Baltscheffsky, M.: Respiration and light-induced phosphorylation in extracts of Rhodospirillum rubrum. J. biol. Chem. 234, 1575–1579 (1959)

  31. van Niel, C. B.: The bacterial photosyntheses and their importance for the general problem of photosynthesis. In: Adv. Enzymol., vol. 1 (F. F. Nord, C. H. Werkman, eds.), pp. 263–328. New York: Interscience Publ. 1941

  32. Vernon, L. P., Kamen, M. D.: Studies on the metabolism of photosynthetic bacteria. XV. Photoautoxidation of ferrocytochrome c in extracts of Rhodospirillum rubrum. Arch. Biochem. Biophys. 44, 298–311 (1953)

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Remennikov, V.G., Samuilov, V.D. Photooxidase activity of isolated chromatophores and intact cells of phototrophic bacteria. Arch. Microbiol. 123, 65–71 (1979). https://doi.org/10.1007/BF00403503

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Key words

  • Phototrophic bacteria
  • Cells
  • Chromatophores
  • Photooxidase activity