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Photooxidase activity of isolated chromatophores and intact cells of phototrophic bacteria

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Abstract

Illumination causes an uptake of oxygen by isolated chromatophores of purple and green bacteria incubated with electron donors. Photooxidase activity of Rhodospirillum rubrum, Chromatium minutissimum, Rhodopseudomonas sphaeroides and Thiocapsa roseopersicina chromatophores is sensitive, and photooxidase activity of Ectothiorhodospira shaposhnikovii and Chlorobium limicola f. thiosulfatophilum is resistant to o-phenanthroline. O2 uptake by illuminated chromatophores of R. rubrum and C. limicola is stimulated upon the increase of pH of incubation mixture from 5 to 9. Photooxidase activity is also manifested in the intact bacterial cells and not merely in the isolated chromatophores. O2 uptake by the illuminated R. rubrum cells treated with CN- is stimulated by 2-heptyl-4-hydroxyquinoline-N-oxide and a protonophorous uncoupler. The interaction of the photosynthetic and respiratory systems of the electron transfer in the bacterial cells and the probable causes of the strong anaerobic way of life of the green sulfur bacteria are discussed.

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Abbreviations

HQNO:

2-heptyl-4-hydroxyquinoline-N-oxide

TMPD:

N,N,-N′,N′-tetramethyl-p-phenylenediamine

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Remennikov, V.G., Samuilov, V.D. Photooxidase activity of isolated chromatophores and intact cells of phototrophic bacteria. Arch. Microbiol. 123, 65–71 (1979). https://doi.org/10.1007/BF00403503

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Key words

  • Phototrophic bacteria
  • Cells
  • Chromatophores
  • Photooxidase activity