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Measurement of chemoautotrophic CO2 assimilation in marine nitrifying bacteria: an enzymatic approach

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Enzyme activities of ribulose-1,5-bisphosphate carboxylase (RUBPCase) and chemoautotrophic CO2 fixation were measured during batch growth of six marine nitrifying bacteria. The NH4-oxidizer Nitrosococcus oceanus and the NO2-oxidizer Nitrococcus mobilis were also examined during exponential growth at different temperatures, O2 tensions and nitrogen-limited growth rates. Cellular activities of RUBPCase and rates of CO2 fixation varied with age in batch culture, temperature, O2 tension and nitrogen limitation, but there was a significant positive correlation between both parameters. Below 26°C, the ratio of CO2 fixation:RUBPCase did not vary significantly with cellular physiological state. Ratios of chemoautotrophic CO2 fixation:RUBCase activity for the six nitrifiers indicated that the ratio was species dependent. Three NO2-oxidizers had a significantly lower CO2 to enzyme ratio than three NH4-oxidizers.

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Literature cited

  1. Belser, L. W. and E. L. Schmidt: Inhibitory effect of nitrapyrin on three genera of ammonium oxidizing nitrifiers. Appl. environ. Microbiol. 41, 819–821 (1981)

  2. Billen, G.: Evaluation of nitrifying activity in sediments by dark 14C-bicarbonate incorporation. Wat. Res. 10, 51–57 (1976)

  3. Billen, G.: A budget of nitrogen recycling in North Sea sediments off the Belgian coast. Estuar. coast. mar. Sci. 7, 127–146 (1978)

  4. Carlucci A. F. and P. M. McNally: Nitrification by marine bacteria in low concentrations of substrate and oxygen. Limnol. Oceanog. 14, 736–739 (1969)

  5. Codispoti, L. A. and T. T. Packard: Denitrification rates in the eastern tropical South Pacific. J. mar. Res. 38, 453–477 (1980)

  6. Eppley, R. W., J. L. Coatsworth and L. Solórzano: Studies of nitrate reductase in marine phytoplankton. Limnol. Oceanog. 14, 195–205 (1969)

  7. Glover, H. E. and I. Morris: Photosynthetic carboxylating enzymes in marine phytoplankton. Limnol. Oceanog. 24, 510–519 (1979)

  8. Goreau, T. J., W. A. Kaplan, S. C. Wofsy, M. B. McElroy, F. W. Valois and S. W. Watson: Production of NO 2 and N2O by nitrifying bacteria at reduced concentrations of oxygen. Appl. environ. Microbiol. 40, 526–532 (1980)

  9. Gundersen, K., A. F. Carlucci and F. Böstrom: Growth of some chemoautotrophic bacteria at different oxygen tensions. Experientia 22, 229–230 (1966)

  10. Hall, G. H.: Apparent and measured rates of nitrification in the hypolimnion of a mesotrophic lake. Appl. environ. Microbiol. 43, 542–547 (1982)

  11. Henriksen, J., I. Hansen and T. H. Blackburn: Rates of nitrification, distribution of nitrifying bacteria and nitrate fluxes in different types of sediment from Danish waters. Mar. Biol. 61, 299–304 (1981)

  12. Horrigan, S. G.: Primary production under the Ross Ice Shelf, Antarctica. Limnol. Oceanog. 26, 378–381 (1981)

  13. Indrebø G., B. Pengerud and I. Dundas: Microbial activities in a permanetly stratified estuary. II. Microbial activities at the oxic-anoxic interface. Mar. Biol. 51, 295–304 (1979)

  14. Kelly, D. P.: Autotrophy: concepts of lithotrophic bacteria and their organic metabolism. Ann. Rev. Microbiol. 25, 177–209 (1971)

  15. Laws, E. A. and J. W. Archie: Appropriate use of regression analysis in marine biology. Mar. Biol. 65, 13–16 (1981)

  16. McCarthy, J. J. and R. W. Eppley: A comparison of chemical, isotopic and enzymatic methods for measuring nitrogen assimilation in marine phytoplankton. Limnol. Oceanog. 17, 371–382 (1972)

  17. Morris, I. and H. E. Glover: Questions on the mechanism of temperature adaptation in marine phytoplankton. Mar. Biol. 24, 147–154 (1974)

  18. Morris, I.: Paths of carbon assimilation in marine phytoplankton. In: Primary productivity in the sea, pp 139–159. Ed. by P. G. Falkowski. New York: Plenum Press 1980

  19. Packard, T. T.: The light dependence of nitrate reductase in marine phytoplankton. Limnol. Oceanog. 18, 466–469 (1973)

  20. Raven, J. A.: Esogenous inorganic carbon sources in plant photosynthesis. Biol. Rev. 45, 167–221 (1970)

  21. Raven, J. A.: Carbon dioxide fixation. In: Algal physiology and biochemistry, pp 434–455. Ed. by W. D. Stewart. London: Blackwell 1974

  22. Someville, M.: A method for the measurement of nitrification rates in water. Wat. Res. 12, 843–848 (1978)

  23. Suess, E., P. M. Müller, N. S. Powell and C. E. Reimers: A closer look at nitrificatin in pelagic sediments. Geochem. J. 14, 129–137 (1980)

  24. Vaccaro, R. F.: Inorganic nitrogen in seawater. In: chemical oceanography, Vol. 1, pp 365–404. Ed. by J. P. Riley and G. Skirrow. New York: Academic Press 1965

  25. Voituriez, B., and A. Herbland: Observation d'un maximum secondaire de nitrite dans l'Atlantique tropical (Dôme de Guinée) Cah. O.R.S.T.O.M. Sér Océanog. 15, 39–46 (1977)

  26. Ward, B. B. and M. J. Perry. Immunofluorescent assay for the marine ammonium oxidizing bacterium Nitrosococcus oceanus. Appl. environ. Micro. 39, 913–918 (1980)

  27. Ward, B. B., R. J. Olson and M. J. Perry: Microbial nitrification rates in the primary nitrite maximum of southern California. Deep-Sea Res. 29, 247–255 (1982)

  28. Watson, S. W.: Marine microbiology five year progress report 1967–1972. W.H.O.I. Doc Collection 46 pp. 1972

  29. Watson, S. W. and M. Mandel: Comparison of the morphology and nucleic acid composition of 27 strains of nitrifying bacteria. J. Bact. 107, 563–569 (1971)

  30. Watson, S. W. and C. C. Remsen: Cell envelope of Nitrosocystis oceanus. J. Ultrastructure Res. 33, 148–160 (1970)

  31. Watson, S. W. and C. C. Waterbury: Characteristics of two marine nitrite oxidizing bacteria, Nitrospina gracilis nov. gen. nov. sp. and Nitrococcus mobilis nov. gen. nov. sp. Arch. Mikrobiol. 77, 203–230 (1971)

  32. Williams, P. J. LeB. and S. W. Watson: Autotrophy in Nitrosocystis oceanus. J. Bact. 96, 1640–1648 (1968)

  33. Yentsch, C. M.: Flow cytometry and cell sorting. Trans. Geophys. Un. 63, 958 (1982)

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Bigelow Laboratory for Ocean Sciences contribution No. 82017

Communicated by S. K. Pierce, College Park

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Glover, H.E. Measurement of chemoautotrophic CO2 assimilation in marine nitrifying bacteria: an enzymatic approach. Mar. Biol. 74, 295–300 (1983). https://doi.org/10.1007/BF00403454

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  • Nitrogen
  • Enzyme
  • Growth Rate
  • Enzyme Activity
  • Assimilation