T- und B-Zellbestimmung in verschiedenen lymphatischen Organen der Maus während der Entstehung N-Nitrosobutyl-Harnstoff-induzierter Lymphome

  • M. Kraus
  • G. R. F. Krueger
Original Papers Experimental Oncology
Received:
Accepted:

Zusammenfassung

In der Latenzzeit eines chemisch induzierten Lymphoms wurde die T- und B-Lymphozytenverteilung im Thymus, der Milz und im Lymphknoten untersucht. N-Nitrosobutyl-Harnstoff wurde in einer Dosierung von 0,04 g/100 ml an 35 Tage alte männliche C57BL-Mäuse 60 Tage lang im Trinkwasser verabreicht. Ab der 11. Woche nach Beginn der Nbu-Verabreichung traten bei 21 von 22 Nbu-Mäusen lymphoblastische Thymuslymphome auf, die sich in der 16. Woche generalisiert in der Milz, im Lymphknoten und in verschiedenen extralymphatischen Organen ausbreiteten. Die immunzytologischen Untersuchungen ließen eine Einordnung dieser Lymphome als reine T-Zell-Lymphome des Thymus mit leukämischer Generalisierung zu. Die Veränderungen des T-Zell-Systems während der Latenzzeit waren durch ein reduziertes Organgewicht, Zellzahlen, sowie eine Verminderung absoluter und relativer T-Zellzahlen des Thymus gekennzeichnet. Ein absoluter T-Zellrückgang fand auch in der Milz statt, obwohl die Verminderung lymphoider Zellzahlen dieses Organes vornehmlich durch den Rückgang der B-Zellzahlen verursacht wurde. Im Lymphknoten traten subnormale Schwankungen theta-positiver Zellen auf, während die B-Zellverteilung unverändert war. Aus den Veränderungen der Lymphozytensubpopulationen in der Latenzzeit des Lymphomwachstums konnte eine Beeinträchtigung der zellulären und humoralen Immunfunktion geschlossen werden. Die Ergebnisse wurden mit murinen Lymphomen anderer Genese verglichen und hinsichtlich einer funktionellen Theorie der Lymphomentwicklung erörtert.

Schlüsselwörter

Chemische Leukämogenese T- und B-Lymphozyten 

T- and B-cell determination in various lymphoid tissues of mice during N-nitrosobutylurea (NBU) leukemogenesis

Summary

T- and B-cell distribution in thymus, spleen, and lymph nodes has been investigated during chemical lymphomagenesis. N-nitrosobutylurea (0.04 g/100 ml) in drinking water was administered to 35-day-old male C57BL mice for a period of 60 days. As of week 11, 21 of 22 Nbu mice developed thymic lymphomas of lymphoblastic cell type which whowed a general leukemic spread to spleen, lymph nodes, and several extralymphatic organs at week 16. Immunocytologic studies revealed the T-cell origin of these thymic type lymphomas. Alteration of the T-cell system during latency period was represented by reduced thymic weight, cell numbers, as well as absolute and relative T-cell reduction. Absolute T-cell reduction was also observed in the spleen, although the reduction of splenic lymphoid cells was mainly caused by a decrease of B cells. In lymph node subnomal variations of theta-positive cells took place, whereas B-cell distribution was not altered. An impaired T- and B-cell function was suspected from preleukemic changes of lymphoid subpopulations. The results are compared with other murine lymphomas and discussed with regard to a functional hypothesis of lymphomagenesis.

Key words

Chemical leukemogenesis T- and B-lymphocytes 
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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • M. Kraus
    • 1
  • G. R. F. Krueger
    • 1
  1. 1.Immunopathology Laboratories, Institute of PathologyUniversity of CologneCologne 41Germany

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