, Volume 172, Issue 1, pp 47–52 | Cite as

Identification of oxindole-3-acetic acid, and metabolic conversion of indole-3-acetic acid to oxindole-3-acetic acid in Pinus sylvestris seeds

  • Arild Ernstsen
  • Göran Sandberg
  • Karin Lundström


Oxindole-3-acetic acid (OxIAA) has been identified in germinating seeds of Scots pine (Pinus sylvestris) using gas chromatography-mass spectrometry. Seeds germinated for 5 d contained 2.7 ng OxIAA·g-1 (dry weight) whereas ungerminated seeds contained 0.2 ng·g-1. Isotopically labelled OxIAA was formed in seeds incubated with [1′-14C]-, [2′-14C]- or [2H5]indole-3-acetic acid.

Key words

Auxin catabolism Indole-3-acetic acid catabolism Pinus Seed germination 



sodium diethyldithiocarbamate


gas chromatography


high-performance liquid chromatography


indole-3-acetic acid


mass spectrometry


oxindole-3-acetic acid






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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Arild Ernstsen
    • 1
  • Göran Sandberg
    • 1
  • Karin Lundström
    • 1
  1. 1.Department of Forest Genetics and Plant PhysiologyThe Swedish University of Agricultural SciencesUmeåSweden

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