Planta

, Volume 170, Issue 4, pp 528–534

d-Mannitol dehydrogenase and d-mannitol-1-phosphate dehydrogenase in Platymonas subcordiformis: some characteristics and their role in osmotic adaptation

  • D. F. E. Richter
  • G. O. Kirst
Article

Abstract

d-Mannitol-1-phosphate dehydrogenase (EC 1.1.1.17) and d-mannitol dehydrogenase (EC 1.1.1.67) were estimated in a cell-free extract of the unicellular alga Platymonas subcordiformis Hazen (Prasinophyceae), d-Mannitol dehydrogenase had two activity maxima at pH 7.0 and 9.5, and a substrate specifity for d-fructose and NADH or for d-mannitol and NAD+. The Km values were 43 mM for d-fructose and 10 mM for d-mannitol. d-Mannitol-1-phosphate dehydrogenase had a maximum activity at pH 7.5 and was specific for d-fructose 6-phosphate and NADH. The Km value for d-fructose 6-phosphate was 5.5 mM. The reverse reaction with d-mannitol 1-phosphate as substrate could not be detected in the extract. After the addition of NaCl (up to 800 mM) to the enzyme assay, the activity of d-mannitol dehydrogenase was strongly inhibited while the activity of d-mannitol-1-phosphate dehydrogenase was enhanced. Under salt stress the Km values of the d-mannitol dehydrogenase were shifted to higher values. The Km value for d-fructose 6-phosphate as substrate for d-mannitol-1-phosphate dehydrogenase remained constant. Hence, it is concluded that in Platymonas the d-mannitol pool is derectly regulated via alternative pathways with different activities dependent on the osmotic pressure.

Key words

d-Mannitol dehydrogenase d-Mannitol-1-phosphate dehydrogenase Osmotic adaptation Platymonas 

Abbreviations

Fru6P

d-fructose 6-phosphate

Mes

2-(N-morpholino)ethanesulfonic acid

MT-DH

d-mannitol-dehydrogenase

MT1P-DH

d-mannitol-1-phosphate dehydrogenase

Pipes

1,4-piperazinediethanesulfonic acid

Tris

2-amino-2-(hydroxymethyl)-1,3-propanediol

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • D. F. E. Richter
    • 1
  • G. O. Kirst
    • 1
  1. 1.Fachbereich BiologieUniversität BremenBremen 33Federal Republic of Germany

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