The alkaline plasmid DNA extraction method of Birnboim and Doly was applied for the isolation of human papillomavirus (HPV) from warts. Tissue from common and plantar warts was digested with proteinase K, and the extrachromosomal circular covalently-closed form of HPV-DNA was rapidly extracted by alkaline sodium dodecyl sulphate and phenol-chloroform treatment. Recovery of HPV-DNA from the tissue was sufficient for determination of endonuclease restriction patterns by agarose gel electrophoresis.
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Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Coggin JR, Zur Hausen H (1979) Workshop on papillomaviruses and cancer. Cancer Res 39:545–546
Doorbar J, Coneron I, Gallimore P (1989) Sequence divergence yet conserved physical characteristics among the E4 proteins of cutaneous human papillomaviruses. Virology 172:51–62
Farve M, Orth G, Croissant O, Yaniv M (1975) Human papillomavirus DNA: physical map. Proc Natl Acad Sci USA 72:4810–4814
Gissmann L, Pfister H, Zur Hausen H (1977) Human papillomaviruses: characterization of four different isolates. J Virol 76:569–580
Gross G, Pfister H, Hargdorn M, Gissmann L (1982) Correlation between human papillomavirus (HPV) type and histology of warts. J Invest Dermatol 78:160–164
Heilman CA, Law M-F, Israel MA, Howley PM (1980) Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J Virol 36:395–407
Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365–369
Kawashima M (1989) On classification of HPV. Hihubyo Shinryo 11:366–372 (in Japanese)
La Porta RF, Taichman LB (1982) Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes. Proc Natl Acad Sci USA 79:3393–3397
Lutzner MA (1983) The human papillomaviruses. Arch Dermatol 119:631–635
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York
McCance DJ (1986) Human papillomaviruses and cancer. Biochem Biophys Acta 823:195–205
McMaster GK, Samulski RJ, Stein GS (1980) Rapid isolation of covalently-closed circular DNAs of bacterial plasmids and animal tumor viruses. Anal Biochem 109:47–54
Orth G, Jabłońska S, Farve M, Croissant O, Obalek S, Jarzabek-Chorzelska M, Jibard N (1981) Identification of human papillomaviruses in Butchers' warts. J Invest Dermatol 76:97–102
Villiers E-M (1989) Heterogeneity of the human papillomaviruses. J Virol 63:4898–4903
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Chinami, M., Tanikawa, E., Hachisuka, H. et al. Human papillomavirus DNA from warts for typing by endonuclease restriction patterns: purification by alkaline plasmid methods. Arch Dermatol Res 282, 446–448 (1990). https://doi.org/10.1007/BF00402620
- Human papillomavirus
- DNA typing