Diabetologia

, Volume 38, Issue 9, pp 1076–1081

A new effective method for the evaluation of glycated intact plasma proteins in diabetic subjects

  • A. Lapolla
  • D. Fedele
  • R. Seraglia
  • S. Catinella
  • L. Baldo
  • R. Aronica
  • P. Traldi
Originals

DOI: 10.1007/BF00402178

Cite this article as:
Lapolla, A., Fedele, D., Seraglia, R. et al. Diabetologia (1995) 38: 1076. doi:10.1007/BF00402178

Summary

The molecular weights of plasma proteins from healthy subjects and from patients with well-or badly-controlled diabetes mellitus have been determined by use of a matrix-assisted laser desorption ionization method, representing a highly accurate technique for the determination of the molecular weight of large biomolecules. Using this approach, different molecular weights of human serum albumin have been found for healthy (66,572–66,694 dalton) and diabetic (66,785–68,959 dalton) subjects. Such differences can be rationalized as being due to the different number of glucose molecules condensed on the protein and/or their further oxidation products; in the case of our diabetic patients this number is in the range of 1.4–14.8. The data show the high validity and specificity of the technique, which allows us to evaluate, without any protein degradation procedure, the number of glucose molecules condensed on a specific protein and ascertain the relationship of this number to the physiopathogenetic conditions of the subjects studied.

Key words

Protein glycation human serum albumin mass spectrometry laser desorption ionization 

Abbreviations

AGE

Advanced glycation end products

FFI

2-(2 furoyl)-4-(5)-(2 furanyl)-1H imidazole

MALDI

matrix-assisted laser desorption/ionization

BSA

bovine serum albumin

HSA

human serum albumin

HbA1c

glycated haemoglobin

Da

dalton

NIDDM

non-insulin-dependent diabetes mellitus

keV

kiloelectron volts

UV

ultraviolet

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. Lapolla
    • 1
  • D. Fedele
    • 1
  • R. Seraglia
    • 2
  • S. Catinella
    • 2
  • L. Baldo
    • 1
  • R. Aronica
    • 1
  • P. Traldi
    • 2
  1. 1.Istituto di Medicina Interna, Cattedra Malattie del MetabolismoUniversità di PadovaPadovaItaly
  2. 2.Area di Ricerca CNRPadovaItaly

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