Advertisement

Archives of Microbiology

, Volume 139, Issue 2–3, pp 225–231 | Cite as

Mycolic acid patterns of some species of Mycobacterium

  • David E. Minnikin
  • S. Megan Minnikin
  • James H. Parlett
  • Michael Goodfellow
  • Mogens Magnusson
Original Papers

Abstract

Representative strains of some species of Mycobacterium were degraded by both acid and alkaline methanolysis. Two-dimensional thin-layer chromatography was used to determine the patterns of mycolic acids and other long-chain components in these methanolysates. Patterns composed of α-, methoxy- and ketomycolates were found in Mycobacterium asiaticum, Mycobacterium bovix, Mycobacterium gastri, Mycobacterium gordonae, Mycobacterium kansasii, Mycobacterium marinum and Mycobacterium tuberculosis; a representative of Mycobacterium thermoresistibile also contained lower molecular weight α′-mycolates in addition to these three acids. In representatives or Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium nonchromogenicum, “Mycobacterium novum”, Mycobacterium paratuberculosis, Mycobacterium scrofulaceum, Mycobacterium terrae, Mycobacterium xenopi, and Mycobacterium sp. MNC 165 α- and ketomycolates were accompanied by ω-carboxymycolates and 2-eicosanol and homologous alcohols which are derived from wax-ester mycolates. Mycobacterium fortuitum and “Mycobacterium giae” contained α′- and epoxymycolates and both serovars of Mycobacterium simiae had a very characteristic pattern of α-, α′- and ketomycolic acids. Comparison with data for other mycobacteria showed the chemotaxonomic significance of these mycolic acid patterns.

Key words

Mycolic acids Mycobacteria Thin-layer chromatographic analysis Acid methanolysates Alka-line methanolysates Chemotaxonomy 

Abbreviations

TLC

thin-layer chromatography

TBDMS

t-butyldimethylsilyl

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Asselineau J (1966) The bacterial lipids. Hermann, ParisGoogle Scholar
  2. Daffé M, Lanéelle MA, Promé D, Asselineau C (1981a) Étude des lipides de mycobacterium gordonae comparativement à ceux de M. leprae et de quelques mycobactéries scotochromogenes. Ann Microbiol (Inst Pasteur) 132B:3–12Google Scholar
  3. Daffé M, Lanéelle MA, Puzo G, asselineau C (1981b) Acide mycolique epoxidique: un nouveau type d'acide mycolique. Tetrahedron Lett 22:4515–4516Google Scholar
  4. Dobson G, Minnikin DE, Parlett JH, Goodfellow M, Ridell M, Magnusson M (1984) Systematic analysis of complex mycobacterial lipids. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, in pressGoogle Scholar
  5. Etémadi AH (1967) Les acides mycoliques, structure, biogenèse et intérêt phylogénétique. Exposés Annuels de Biochimie Médicale 28:77–109Google Scholar
  6. Goodfellow M, Minnikin DE (1981) Identification of Mycobacterium chelonei by thin-layer chromatographic analysis of whole-organism methanolysates. Tubercle 62:285–287Google Scholar
  7. Goodfellow M, Wayne LG (1982) Taxonomy and nomenclature. In: Ratledge C, Stanford JL (eds). The biology of the mycobacteria, Academic Press, London, pp 471–521Google Scholar
  8. Lévy-Frébault V, Rafidinarivo E, Promé JC, Grandry J, Boisvert H, David HL (1983a) Mycobacterium fallax sp. nov. Int J Syst Bacteriol 33:336–343Google Scholar
  9. Lévy-Frébault V, Daffé M, Seng Goh K, Lanéelle MA, Asselineau C, David HL (1983b) Identification of Mycobacterium fortuitum and Mycobacterium chelonei. J Clin Microbiol 17:744–572Google Scholar
  10. Magnusson M (1981) Mycobacterial sensitins: where are we now? Rev Infect Dis 3:944–948Google Scholar
  11. Meissner G, Schröder KH (1975) Relationship between Mycobacterium simiae and Mycobacterium habana. Am Rev Respir Dis 111:196–200Google Scholar
  12. Meissner G, Schröder KH, Amadio GE, Anz W, Chaparas S, Engel HWB, Jenkins PA, Käppler W, Kleeberg HH, Kubala E, Kubin M, Lauterbach D, Lind A, Magnusson M, Miková ZD, Pattyn SR, Schaefer WB, Stanford JL, Tsukamura M, Wayne LG, Willers I, Wolinsky E (1974) A co-operative analysis of nonscoto- and nonphoto-chromogenic slowly growing mycobacteria. J Gen Microbiol 83:207–235Google Scholar
  13. Minnikin DE (1982) Lipids: complex lipids, their chemistry, biosynthesis and roles. In: Ratledge C, Stanford JL (eds) The biology of the mycobacteria. Academic Press, London, pp 95–184Google Scholar
  14. Minnikin DE, Goodfellow M (1980) Lipid composition in the classification and identification of acid-fast bacteria. In Goodfellow M, Board RG (eds) Microbiological classification and identification. Academic Press, London, pp 189–256Google Scholar
  15. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233Google Scholar
  16. Minnikin DE, Minnikin SM, Goodfellow M (1982a) The oxygenated mycolic acids of Mycobacterium fortuitum, M. farcinogenes and M. senegalense. Biochim Biophys Acta 712:616–620Google Scholar
  17. Minnikin DE, Minnikin SM, Goodfellow M, Stanford JL (1982b) The mycolic acids of Mycobacterium chelonei. J Gen Microbiol 128:817–822Google Scholar
  18. Minnikin DE, Minnikin SM, O'Donnell AG, Goodfellow M (1984a) Extraction of mycobacterial mycolic acids and other long-chain compounds by an alkaline methanolysis procedure. J Microbiol Methods 2:243–249Google Scholar
  19. Minnikin DE, Minnikin SM, Hutchinson IG, Goodfellow M, Grange JM (1984b) Mycolic acid patterns of representative strains of Mycobacterium fortuitum, “Mycobacterium peregrinum” and Mycobacterium smegmatis. J Gen Microbiol 130:363–367Google Scholar
  20. Runyon EH, Wayne LG, Kubica GP (1974) Mycobacterium Lehmann and Neumann. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of determinative bacteriology, 8th ed. The Williams and Wilkins Co., Baltimore, pp 681–701Google Scholar
  21. Skerman VBD, McGowan V, Sneath PHA (1980) Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420Google Scholar
  22. Tsukamura M, Kita N, Otsuka W, Shimoide H (1983) A study of the taxonomy of the Mycobacterium nonchromogenicum complex and report of six cases of lung infection due to Mycobacterium nonchromogenicum. Microbiol Immunol 27:219–236Google Scholar
  23. Wolinsky E, Schaefer WB (1975) Proposed numbering scheme for mycobacterial serotypes by agglutination. Int J Syst Bacteriol 23:182–183Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • David E. Minnikin
    • 1
  • S. Megan Minnikin
    • 1
  • James H. Parlett
    • 1
    • 2
  • Michael Goodfellow
    • 1
    • 2
  • Mogens Magnusson
    • 3
  1. 1.Department of Organic ChemistryThe UniversityNewcastle upon TyneUK
  2. 2.Department of MicrobiologyThe UniversityNewcastle upon TyneUK
  3. 3.Tuberculin DepartmentStatens SeruminstitutCopenhagenDenmark

Personalised recommendations