Marine Biology

, Volume 61, Issue 4, pp 267–276 | Cite as

Uptake and accumulation of naphthalene by the oyster Ostrea edulis, in a flow-through system

  • R. T. Riley
  • M. C. Mix
  • R. L. Schaffer
  • D. L. Bunting
Article

Abstract

A flow-through system was used to follow naphthalene and naphthalene metabolite accumulation in the seawater and in the tissue of the oyster Ostrea edulis. After 72 h, 82.5% of the naphthalene carbon was recovered from the system. Glucose was added to seawater to stimulate the pathways of glucose metabolism in the oysters. Streptomycin (100 ppm) reduced microbial oxidation of naphthalene and glucose, and reduced bacterial growth. However, even in the presence of streptomycin, microbial oxidation of naphthalene was considerable. The main oxidation product recovered from seawater was 14CO2. Radioactivity was also associated with compounds which separated by TLC with 2- and 1- naphthol. The pattern of naphthalene uptake and accumulation in oyster tissues was relatively constant after only a few hours of exposure to naphthalene. The potential of tissues to accumulate naphthalene was shown to be a function of multiple variables such as nutritional state, lipid concentration, length of exposure to naphthalene, and the external naphthalene concentration. Carbon-14-labeled metabolites derived from 14C-naphthalene were consistently recovered from digests of the oyster tissues. Non-CO2 alkaline-soluble substances were the primary metabolites. Hexane-extractable substances, which separated by TLC with known standards of 2- and 1- naphthol, were consistently recovered from seawater and tissue digests. It was not possible to conclude that these metabolites were a result of naphthalene metabolism by oyster enzyme systems.

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Literature Cited

  1. Anderson, J. W., J. M. Neff, B. A. Cox, H. E. Tatem and C. M. Hightower: Characteristics of dispersions and watersoluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. Mar. Biol. 27, 75–88 (1974)Google Scholar
  2. Anderson, R. S.: Benzo[a]pyrene metabolism in the American oyster Crassostrea virginica. Ecol. Res. Ser. 1–19 (1978). (U.S. Environmental Protection Agency, Environmental Research Laboratory, Gulf Breeze, Florida 32561, USA. Ref.: EPA-600/3-78-009)Google Scholar
  3. Bend, J. R., M. O. James, and P. M. Dansette: In vitro metabolism of xenobiotics in some marine animals. Ann. N. Y. Acad. Sci. 298, 505–521 (1977)Google Scholar
  4. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith: Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28, 350–356 (1956)Google Scholar
  5. Folch, J., N. Lees and C. H. Sloane-Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957)PubMedGoogle Scholar
  6. Fong, W. C.: Uptake and retention of Kuwait crude oil and its effect on oxygen uptake by the soft-shell clam, Mya arenaria. J. Fish. Res. Bd Can. 33, 2774–2780 (1976)Google Scholar
  7. Lee, R. F., R. Sauerheber and A. A. Benson: Petroleum hydrocarbons: uptake and discharge by the marine mussel Mytilus edulis. Science, N. Y. 177, 344–346 (1972)Google Scholar
  8. Marsh, J. B. and D. B. Weinstein: Simple charring method for determination of lipids. J. Lipid Res. 7, 574–576 (1966)PubMedGoogle Scholar
  9. Péquignat, E.: A kinetic and autoradiographic study of the direct assimilation of amino acids and glucose by organs of the mussel Mytilus edulis. Mar. Biol. 19, 227–244 (1973)Google Scholar
  10. Peterson, R. G.: Exercise in statistical inference, 258 pp. Corvallis: Oregon State University Press 1973Google Scholar
  11. Rice, S. D., J. W. Short and J. F. Karinen: Toxicity of Cook Inlet crude oil and no. 2 fuel oil to several Alaskan marine fish and invertebrates. In: Proceedings of Symposium on Sources, Effects and Sinks of Hydrocarbons in the Aquatic Environment, pp 394–406. Arlington, Virginia: American Institute of Biological Sciences 1976Google Scholar
  12. Roubal, W. T., T. K. Collier and D. C. Malins: Accumulation and metabolism of carbon-14 labeled benzene, naphthalene, and anthracene by young Coho salmon (Oncorhynchus kisutch). Arch. envir. Contam. Toxicol. 5, 513–523 (1977)Google Scholar
  13. Stainken, D. M.: Preliminary observations on the mode of accumulation of #2 fuel oil by the soft shell clam, Mya arenaria. Proc. Conf. Prev. Control Oil Pollut. 1975, 463–468 (1975). (Washington, D. C.: American Petroleum Institute)Google Scholar
  14. Stegeman, J. J. and J. M. Teal: Accumulation, release and retention of petroleum hydrocarbons by the oyster Crassostrea virginica. Mar. Biol. 22, 37–44 (1973)Google Scholar
  15. Stevenson, H. L., C. E. Millwood and B. H. Hebeler: Aerobic, heterotrophic bacterial populations in estuarine water and sediment. In: Effects of the ocean environment on microbial activities pp 268–285. Ed. by R. R. Colwell and R. Y. Morita. Baltimore: University Park Press 1974Google Scholar
  16. Tinsley, I. J.: Chemical concepts in pollutant behavior, 265 pp. New York: Wiley-Interscience 1979Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • R. T. Riley
    • 1
  • M. C. Mix
    • 1
  • R. L. Schaffer
    • 1
  • D. L. Bunting
    • 1
  1. 1.Department of General ScienceOregon State UniversityCorvallisUSA
  2. 2.Toxicology and Biological Constituents Research UnitRussell Research CenterAthensUSA

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