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Diabetologia

, Volume 35, Issue 12, pp 1177–1180 | Cite as

Microdialysis measurement of the absolute glucose concentration in subcutaneous adipose tissue allowing glucose monitoring in diabetic patients

  • J. Bolincier
  • U. Ungerstedt
  • P. Arner
Rapid Communication

Summary

The possibility of continuously monitoring the absolute glucose concentration in subcutaneous adipose tissue, using microdialysis of the extracellular water space, was investigated in six Type 1 (insulin-dependent) diabetic patients. By using a large microdialysis probe (30×0.62 mm), and by perfusing with a low flow rate (0.5 μl/min), complete recovery of glucose was attained in vitro. In the patients the dialysis probe was implanted subcutaneously, perfused by a wearable microinfusion pump, and dialysate samples were collected in 60-min fractions over 10 h. The absolute glucose concentration in the tissue dialysate was the same or almost the same as the blood glucose concentration (range 87–101 % of the blood glucose value). The changes in blood glucose were closely paralleled by the variations in adipose tissue glucose (r=0.93, p<0.01), and the recovery of glucose in the microdialysate remained constant during the 10-h study period. In conclusion, it is possible, using microdialysis, to directly determine the absolute glucose concentration in subcutaneous adipose tissue. Hence, this technique may be used for continuous glucose monitoring in diabetic patients.

Key words

Microdialysis glucose monitoring subcutaneous adipose tissue 

References

  1. 1.
    Fischer U, Ertle R, Abel P et al. (1987) Assessment of subcutaneous glucose concentration: validation of the wick technique as a reference for implanted electrochemical sensors in normal and diabetic dogs. Diabetologia 30: 940–945CrossRefPubMedGoogle Scholar
  2. 2.
    Pickup JC (1985) Biosensors: a clinical perspective. Lancet II: 817–820CrossRefGoogle Scholar
  3. 3.
    Arner P, Bolinder J (1991) Microdialysis of adipose tissue. J Intern Med 230: 381–386PubMedGoogle Scholar
  4. 4.
    Ungerstedt U (1991) Microdialysis — principles and applications for studies in animal and man. J Intern Med 230: 365–373PubMedGoogle Scholar
  5. 5.
    Tossman U, Ungerstedt U (1986) Microdialysis in the study of extracellular levels of amino acids in the rat brain. Acta Physiol Scand 128: 9–14PubMedGoogle Scholar
  6. 6.
    Kaddish AH, Little RL, Sternberg JC (1968) A new and rapid method for the determination of glucose measurement of the rate of oxygen consumption. Clin Chem 14: 116–131Google Scholar
  7. 7.
    Lönnroth P, Jansson P-A, Smith U (1987) A microdialysis method allowing characterization of intercellular water space in humans. Am J Physiol 253: E228-E231PubMedGoogle Scholar
  8. 8.
    Jansson P-A, Fowelin J, Smith U, Lönnroth P (1988) Characterization by microdialysis of intercellular glucose levels in subcutaneous tissue in humans. Am J Physiol 255: E218-E220PubMedGoogle Scholar
  9. 9.
    Bolinder J, Hagström E, Ungerstedt U, Arner P (1989) Microdialysis of subcutaneous adipose tissue in vivo for continuous glucose monitoring in man. Scand J Clin Lab Invest 49: 465–474PubMedGoogle Scholar
  10. 10.
    Hagström-Toft E, Arner P, Näslund B, Ungerstedt U, Bolinder J (1991) Effects of insulin deprivation and replacement on in vivo subcutaneous adipose tissue substrate metabolism in humans. Diabetes 40: 666–672PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Bolincier
    • 1
  • U. Ungerstedt
    • 2
  • P. Arner
    • 1
  1. 1.Department of Medicine and the Research Center at Huddinge HospitalStockholmSweden
  2. 2.Department of PharmacologyKarolinska InstituteStockholmSweden

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